high-yield hydrogen production from starch and water by a synthetic enzymatic pathway从淀粉高产氢和水合成的酶通路.pdfVIP

High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway 1 2 3 4 4 Y.-H. Percival Zhang *, Barbara R. Evans , Jonathan R. Mielenz , Robert C. Hopkins , Michael W. W. Adams 1 Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia, United States of America, 2 Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America, 3 Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America, 4 Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, United States of America Background. The future hydrogen economy offers a compelling energy vision, but there are four main obstacles: hydrogen production, storage, and distribution, as well as fuel cells. Hydrogen production from inexpensive abundant renewable biomass can produce cheaper hydrogen, decrease reliance on fossil fuels, and achieve zero net greenhouse gas emissions, but current chemical and biological means suffer from low hydrogen yields and/or severe reaction conditions. Methodology/ Principal Findings. Here we demonstrate a synthetic enzymatic pathway consisting of 13 enzymes for producing hydrogen from starch and water. The stoichiometric reaction is C H O (l)+7 H O (l)R 12 H (g)+6 CO (g). The overall process is 6 10 5 2 2 2 spontaneous and unidirectional because of a negative Gibbs free energy and separation of the gaseous products with the aqueous reactants. Conclusions. Enzymatic hydrogen production from starch and water mediated by 13 enzymes occurred at 30uC as expected, and the hydrogen