hpv e6e7 mrna testing is more specific than cytology in post-colposcopy follow-up of women with negative cervical biopsyhpv e6e7 mrna比细胞学post-colposcopy后续的更具体的测试是消极的女性宫颈活检.pdfVIP

HPV E6/E7 mRNA Testing Is More Specific than Cytology in Post-Colposcopy Follow-Up of Women with Negative Cervical Biopsy 1 2 1 3,4 Sveinung Wergeland Sørbye *, Marc Arbyn , Silje Fismen , Tore Jarl Gutteberg , Elin Synnøve Mortensen1,4 1 Department of Clinical Pathology, The University Hospital of North Norway, Tromsø, Norway, 2 Unit of Cancer Epidemiology, Scientific Institute of Public Health, Brussels, Belgium, 3 Department of Microbiology and Infection Control, The University Hospital of North Norway, Tromsø, Norway, 4 Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway Abstract Background: In Norway, women with negative or low-grade cervical biopsies (normal/CIN1) are followed up after six months in order to decide on further follow-up or recall for screening at three-year intervals. A high specificity and positive predictive value (PPV) of the triage test is important to avoid unnecessary diagnostic and therapeutic procedures whereas a low risk of high-grade disease among triage negative women assures safety. Materials and Methods: At the University Hospital of North Norway, cytology and the HPV mRNA test PreTect HPV-Proofer, detecting E6/E7 mRNA from HPV types 16, 18, 31, 33 and 45, are used in post-colposcopy follow-up of women with negative or low-grade biopsy. In this study, women with negative biopsy after high grade cytology (ASC-H/HSIL) and/or positive HPV mRNA test in the period 2005–2009 were included (n = 520). Histologically confirmed cervical intraepithelial neoplasia of grade 2 or worse (CIN2+) was used as study endpoint. Results: Of 520 women with negative or low-grade biopsy, 124 women (23.8%) had CI