high prevalence of human parvovirus 4 infection in hbv and hcv infected individuals in shanghai人类细小病毒高发4感染乙肝病毒和丙肝病毒感染者在上海.pdfVIP

High Prevalence of Human Parvovirus 4 Infection in HBV and HCV Infected Individuals in Shanghai 1 3 3 1 2 1. 4 . Xuelian Yu , Jing Zhang , Liang Hong , Jiayu Wang , Zhengan Yuan , Xi Zhang , Reena Ghildyal * 1 Microbiology Laboratory, Hongkou District Center for Disease Control and Prevention, Shanghai, People’s Republic of China, 2 Shanghai Municipal Center for Disease Control and Prevention, Hongkou District Center for Disease Control and Prevention, Shanghai, People’s Republic of China, 3 Microbiology Laboratory, Hongkou District Center for Disease Control and Prevention, Shanghai, People’s Republic of China, 4 Respiratory Virology Group, Centre for Research in Therapeutic Solutions, Faculty of Applied Science, University of Canberra, Canberra, Australia Abstract Human parvovirus 4 (PARV4) has been detected in blood and diverse tissues samples from HIV/AIDS patients who are injecting drug users. Although B19 virus, the best characterized human parvovirus, has been shown to co-infect patients with hepatitis B or hepatitis C virus (HBV, HCV) infection, the association of PARV4 with HBV or HCV infections is still unknown. The aim of this study was to characterise the association of viruses belonging to PARV4 genotype 1 and 2 with chronic HBV and HCV infection in Shanghai. Serum samples of healthy controls, HCV infected subjects and HBV infected subjects were retrieved from Shanghai Center for Disease Control and Prevention (SCDC) Sample Bank. Parvovirus-specific nested-PCR was performed and results confirmed by sequencing. Sequences were compared with reference sequences obtained from Genbank to derive phylogeny trees. The