hrpa, a deah-box rna helicase, is involved in global gene regulation in the lyme disease spirochetehrpa deah-box rna解旋酶是参与全球莱姆病螺旋体的基因调控.pdfVIP

HrpA, a DEAH-Box RNA Helicase, Is Involved in Global Gene Regulation in the Lyme Disease Spirochete Aydan Salman-Dilgimen, Pierre-Olivier Hardy, Ashley R. Dresser, George Chaconas* Departments of Biochemistry and Molecular Biology and Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada Abstract Spirochetes causing Lyme borreliosis are obligate parasites that can only be found in a tick vector or a vertebrate host. The ability to survive in these two disparate environments requires up and downregulation of specific genes by regulatory circuits that remain largely obscure. In this work on the Lyme spirochete, B. burgdorferi, we show that a disruption of the hrpA gene, which encodes a putative RNA helicase, results in a complete loss in the ability of the spirochetes to infect mice by needle inoculation. Studies of protein expression in culture by 2D gels revealed a change in the expression of 33 proteins in hrpA clones relative to the wild-type parent. Quantitative characterization of protein expression by iTRAQ analysis revealed a total of 187 differentially regulated proteins in an hrpA background: 90 downregulated and 97 upregulated. Forty-two of the 90 downregulated and 65 of the 97 upregulated proteins are not regulated under any conditions by the previously reported regulators in B. burgdorferi (bosR, rrp2, rpoN, rpoS or rrp1). Downregulated and upregulated proteins also fell into distinct functional categories. We conclude that HrpA is part of a new and distinct global regulatory pathway in B. burgdorferi gene expression. Because an HrpA orthologue is present in many bacteria, its participation in global regulation in B. burgdorferi may have relevance in other bacterial species where its function remains obscure. We believe this to be the first report of a role for an RNA helicase in a global regu