hiv-1 with multiple ccr5cxcr4 chimeric receptor use is predictive of immunological failure in infected childrenhiv - 1与多个ccr5cxcr4嵌合受体使用预测受感染儿童的免疫失败.pdfVIP

HIV-1 with Multiple CCR5/CXCR4 Chimeric Receptor Use Is Predictive of Immunological Failure in Infected Children 1 2 3 4 5 Mariangela Cavarelli *, Ingrid Karlsson , Marisa Zanchetta , Liselotte Antonsson , Anna Plebani , Carlo 6 ¨ 2 3 1 Giaquinto , Eva Maria Fenyo , Anita De Rossi , Gabriella Scarlatti 1Viral Evolution and Transmission Unit, DIBIT, Fondazione Centro San Raffaele, Milan, Italy, 2 Division of Medical Microbiology/Virology, Department of Laboratory Medicine, Lund University, Lund, Sweden, 3 Department of Oncology and Surgical Sciences, Unit of Viral Oncology, AIDS Reference Center, University of Padova, IOV- IRCCS, Padova, Italy, 4 Division of Cellular and Molecular Pharmacology, Department of Experimental Medical Science, Lund University, Lund, Sweden, 5 Department of Pediatrics, University of Milan, Clinica De Marchi, Milan, Italy, 6 Department of Pediatrics, University of Padova, Padova, Italy Abstract Background: HIV-1 R5 viruses are characterized by a large phenotypic variation, that is reflected by the mode of coreceptor use. The ability of R5 HIV-1 to infect target cells expressing chimeric receptors between CCR5 and CXCR4 (R5broad viruses), was shown to correlate with disease stage in HIV-1 infected adults. Here, we ask the question whether phenotypic variation of R5 viruses could play a role also in mother-to-child transmission (MTCT) of HIV-1 and pediatric disease progression. Methodology/Principal Findings: Viral isolates obtained from a total of 59 HIV-1 seropositive women (24 transmitting and 35 non transmitting) and 28 infected newborn children, were used to infect U87.CD4 cells expressing wild type or six