growth-rate dependence reveals design principles of plasmid copy number control经济增长依赖揭示了质粒拷贝数控制的设计原则.pdfVIP

Growth-Rate Dependence Reveals Design Principles of Plasmid Copy Number Control Stefan Klumpp1,2* 1 Max Planck Institute of Colloids and Interfaces, Potsdam, Germany, 2 Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California, United States of America Abstract Genetic circuits in bacteria are intimately coupled to the cellular growth rate as many parameters of gene expression are growth-rate dependent. Growth-rate dependence can be particularly pronounced for genes on plasmids; therefore the native regulatory systems of a plasmid such as its replication control system are characterized by growth-rate dependent parameters and regulator concentrations. This natural growth-rate dependent variation of regulator concentrations can be used for a quantitative analysis of the design of such regulatory systems. Here we analyze the growth-rate dependence of parameters of the copy number control system of ColE1-type plasmids in E. coli. This analysis allows us to infer the form of the control function and suggests that the Rom protein increases the sensitivity of control. Citation: Klumpp S (2011) Growth-Rate Dependence Reveals Design Principles of Plasmid Copy Number Control. PLoS ONE 6(5): e20403. doi:10.1371/ journal.pone.0020403 Editor: Christina Chan, Michigan State University, United States of America Received February 22, 2011; Accepted April 25, 2011; Published May 27, 2011 Copyright: 2011 Stefan Klumpp. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The initial phase of this work was supported by Deutsche Forschungsgemeinschaft (fellowship KL818/1-1 and 1-2), by the National Science Foundation (NSF