gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults肠道微生物群在成人2型糖尿病患者与非糖尿病成年人.pdfVIP

Gut Microbiota in Human Adults with Type 2 Diabetes Differs from Non-Diabetic Adults 1 1 1 1 Nadja Larsen *, Finn K. Vogensen , Frans W. J. van den Berg , Dennis Sandris Nielsen , Anne Sofie 2 2 3 3 3 Andreasen , Bente K. Pedersen , Waleed Abu Al-Soud , Søren J. Sørensen , Lars H. Hansen , Mogens Jakobsen1 1 Department of Food Science, University of Copenhagen, Frederiksberg, Denmark, 2 Department of Infectious Diseases and CMRC, University Hospital Rigshospitalet, Copenhagen, Denmark, 3 Department of Biology, University of Copenhagen, Copenhagen, Denmark Abstract Background: Recent evidence suggests that there is a link between metabolic diseases and bacterial populations in the gut. The aim of this study was to assess the differences between the composition of the intestinal microbiota in humans with type 2 diabetes and non-diabetic persons as control. Methods and Findings: The study included 36 male adults with a broad range of age and body-mass indices (BMIs), among which 18 subjects were diagnosed with diabetes type 2. The fecal bacterial composition was investigated by real-time quantitative PCR (qPCR) and in a subgroup of subjects (N = 20) by tag-encoded amplicon pyrosequencing of the V4 region of the 16S rRNA gene. The proportions of phylum Firmicutes and class Clostridia were significantly reduced in the diabetic group compared to the control group (P = 0.03). Furthermore, the ratios of Bacteroidetes to Firmicutes as well as the ratios of Bacteroides-Prevotella group to C. coccoides-E. rectale group correlated positively and significantly with plasma glucose concentration (P = 0.04)