有哪些信誉好的足球投注网站genotypic and phenotypic characterization of chikungunya virus of different genotypes from malaysia齐昆古尼亚病毒基因型和表型鉴定从马来西亚不同的基因型.pdfVIP
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genotypic and phenotypic characterization of chikungunya virus of different genotypes from malaysia齐昆古尼亚病毒基因型和表型鉴定从马来西亚不同的基因型
Genotypic and Phenotypic Characterization of Chikungunya Virus of Different Genotypes from Malaysia 1 1 1 1 I-Ching Sam *, Shih-Keng Loong , Jasmine Chandramathi Michael , Chong-Long Chua , Wan Yusoff Wan 2 2 1 1 2 Sulaiman , Indra Vythilingam , Shie-Yien Chan , Chun-Wei Chiam , Yze-Shiuan Yeong , Sazaly AbuBakar1, Yoke-Fun Chan1 1Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia, 2 Department of Parasitology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia Abstract Background: Mosquito-borne Chikungunya virus (CHIKV) has recently re-emerged globally. The epidemic East/Central/ South African (ECSA) strains have spread for the first time to Asia, which previously only had endemic Asian strains. In Malaysia, the ECSA strain caused an extensive nationwide outbreak in 2008, while the Asian strains only caused limited outbreaks prior to this. To gain insight into these observed epidemiological differences, we compared genotypic and phenotypic characteristics of CHIKV of Asian and ECSA genotypes isolated in Malaysia. Methods and Findings: CHIKV of Asian and ECSA genotypes were isolated from patients during outbreaks in Bagan Panchor in 2006, and Johor in 2008. Sequencing of the CHIKV strains revealed 96.8% amino acid similarity, including an unusual 7 residue deletion in the nsP3 protein of the Asian strain. CHIKV replication in cells and Aedes mosquitoes was measured by virus titration. There were no diffe
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