有哪些信誉好的足球投注网站genotypic diversity and drug susceptibility patterns among m. tuberculosis complex isolates from south-western ghana结核分枝杆菌基因型多样性和药敏模式之间复杂的隔离从西南加纳.pdfVIP
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genotypic diversity and drug susceptibility patterns among m. tuberculosis complex isolates from south-western ghana结核分枝杆菌基因型多样性和药敏模式之间复杂的隔离从西南加纳
Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South- Western Ghana 1 1 2 4,5 1 Dorothy Yeboah-Manu *, Adwoa Asante-Poku , Thomas Bodmer , David Stucki , Kwadwo Koram , Frank Bonsu3, Gerd Pluschke5,6, Sebastien Gagneux4,5,6 1 Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana, 2 University of Bern, Institute for Infectious Diseases, Bern, Switzerland, 3 Ghana Health Services, Accra, Ghana, 4 MRC, National Institute for Medical Research, London, United Kingdom, 5 Swiss Tropical and Public Health Institute, Basel, Switzerland, 6 University of Basel, Basel, Switzerland Abstract Objective: The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates. Methods: MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Isolates were further typed using spoligotyping, and the phenotypic drug susceptibility patterns were determined by the proportion method. Result: One hundred and sixty-two isolates were characterised by LSP typing. Of these, 130 (80.25%) were identified as Mycobacterium tuberculosis sensu stricto (MTBss), with the Cameroon sub-lineage being dominant (N = 59/130, 45.38%). Thirty-two (19.75%) isolates were classified as Mycobacterium africanum type 1, and of these 26 (81.25%) were identified as West-Africa I, and 6 (18.75%) as West-Africa II. Spoligotyping sub-lineages identified among the MTBss included Haarlem (N = 15, 11.53%), Ghana (N = 22, 16.92%), Beijing (4, 3.08%), EA
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