genotypic variation and mixtures of lyme borrelia in ixodes ticks from north america and europe基因型变异和混合物的莱姆包柔氏螺旋体ixodes蜱虫从北美和欧洲.pdfVIP

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genotypic variation and mixtures of lyme borrelia in ixodes ticks from north america and europe基因型变异和混合物的莱姆包柔氏螺旋体ixodes蜱虫从北美和欧洲.pdf

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genotypic variation and mixtures of lyme borrelia in ixodes ticks from north america and europe基因型变异和混合物的莱姆包柔氏螺旋体ixodes蜱虫从北美和欧洲

Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe 1 1 5 1 3 Chris D. Crowder , Heather E. Matthews , Steven Schutzer , Megan A. Rounds , Benjamin J. Luft , Oliver 4 2 1 1 1 1 Nolte , Scott R. Campbell , Curtis A. Phillipson , Feng Li , Ranga Sampath , David J. Ecker , Mark W. Eshoo1* 1 Ibis Biosciences, Carlsbad, California, United States of America, 2 Suffolk County Department of Health Services, Yaphank, New York, United States of America, 3 Department of Medicine, State University of New York at Stony Brook, Stony Brook, New York, United States of America, 4 Laboratory of Dr. Brunner, Constance, Germany, 5 Department of Medicine, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, United States of America Abstract Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America and Europe. Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of