genetic manipulation of palmitoylethanolamide production and inactivation in saccharomyces cerevisiae在酿酒酵母基因操纵palmitoylethanolamide生产和失活.pdfVIP

Genetic Manipulation of Palmitoylethanolamide Production and Inactivation in Saccharomyces cerevisiae 1,2 3 3 1,4 Giulio G. Muccioli , Angela Sia , Paul J. Muchowski *, Nephi Stella * 1 Department of Pharmacology, University of Washington, Seattle, Washington, United States of America, 2 Louvain Drug Research Institute, Bioanalysis and ´ Pharmacology of Bioactive Lipids Laboratory, Chemical and Physico-chemical Analysis of Drugs Unit, UCL-CHAM (7230), Universite catholique de Louvain, Bruxelles, Belgium, 3 Gladstone Institute of Neurological Disease and Departments of Biochemistry and Biophysics, and Neurology, University of California San Francisco, San Francisco, California, United States of America, 4 Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, Washington, United States of America Abstract Background: Lipids can act as signaling molecules, activating intracellular and membrane-associated receptors to regulate physiological functions. To understand how a newly discovered signaling lipid functions, it is necessary to identify and characterize the enzymes involved in their production and inactivation. The signaling lipid N-palmitoylethanolamine (PEA) is known to activate intracellular and membrane-associated receptors and regulate physiological functions, but little is known about the enzymes involved in its production and inactivation. Principal Findings: Here we show that Saccharomyces cerevisiae produce and inactivate PEA, suggesting that genetic manipulations of this lower