genotypic and pfgemlva analyses of vibrio cholerae o1 geographical spread and temporal changes during the 2007–2010 cholera outbreaks in thailand基因型和pfgemlva分析霍乱弧菌o1群的地理分布和时间变化在2007 - 2010年在泰国霍乱暴发.pdfVIP

下载本文档

1
0
约7.36万字
约 10页
2017-09-01 发布于上海
举报
版权申诉

genotypic and pfgemlva analyses of vibrio cholerae o1 geographical spread and temporal changes during the 2007–2010 cholera outbreaks in thailand基因型和pfgemlva分析霍乱弧菌o1群的地理分布和时间变化在2007 - 2010年在泰国霍乱暴发.pdf

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

genotypic and pfgemlva analyses of vibrio cholerae o1 geographical spread and temporal changes during the 2007–2010 cholera outbreaks in thailand基因型和pfgemlva分析霍乱弧菌o1群的地理分布和时间变化在2007 - 2010年在泰国霍乱暴发

Genotypic and PFGE/MLVA Analyses of Vibrio cholerae O1: Geographical Spread and Temporal Changes during the 2007–2010 Cholera Outbreaks in Thailand 1,2 1 3 1,2 Kazuhisa Okada *, Amonrattana Roobthaisong , Ichiro Nakagawa , Shigeyuki Hamada , Siriporn Chantaroj4 1Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi, Thailand, 2 Research Collaboration Center on Emerging and Re- emerging Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan, 3 Section of Bacterial Pathogenesis, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan, 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand Abstract Background: Vibrio cholerae O1 El Tor dominated the seventh cholera pandemic which occurred in the 1960s. For two decades, variants of V. cholerae O1 El Tor that produce classical cholera toxin have emerged and spread globally, replacing the prototypic El Tor biotype. This study aims to characterize V. cholerae O1 isolates from outbreaks in Thailand with special reference to genotypic variations over time. Methods/Findings: A total of 343 isolates of V. cholerae O1 from cholera outbreaks from 2007 to 2010 were investigated, and 99.4% were found to carry the classical cholera toxin B subunit (ctxB) and El Tor rstR genes. Pulsed-field gel electrophoresis (PFGE) differentiated the isolates into 10 distinct pulsotypes, clustered into two major groups, A and B, with an overall similarity of 88%. Ribotyping, multiple-locus variable-number tandem-repeat analysis (MLVA), and PCR to detect Vibrio seven