一氧化氮对舌下神经核兴奋作用影响.docVIP

一氧化氮对舌下神经核兴奋作用影响[摘要] 目的 观察一氧化氮（NO）及其清除剂对舌下神经运动核（HMN）活动的影响并初步探讨其机制。方法 麻醉雄性成年Wistar大鼠，微透析探针先透析人工脑脊液（ACSF）到HMN作为对照，然后再分别持续给予二乙胺一氧化氮水合钠盐（DEA，NO供体）和2-（4-羧基苯基）-4,4,5,5,-四甲基-1,3-二氧咪唑啉钾盐（carboxy-PTIO，NO清除剂）到HMN，观测大鼠颏舌肌（GG）、膈肌电活动及血压和呼吸频率的变化。结果 给予DEA后，大鼠的呼吸相关GG活动明显增加，而给予carboxy-PTIO后则明显降低，与ACSF时的值相比差异均有统计学意义（P0.05）。结论 NO作为一种神经递质对HMN产生兴奋，从而对维持生理状态下上气道的开放起重要作用。 [关键词] 一氧化氮； 舌下神经核； 神经递质； 颏舌肌； 阻塞性睡眠呼吸暂停综合征 [中图分类号] Q 47 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.03.023 Nitric oxide acts as an excitatory neurotransmitter at hypoglossal motor nucleus Wu Haiying1, Liu Hattie2, Horner Richard L2, Liu Liu3. （1. Dept. of Otolaryngology, The Second Affiliated Hospital of Kunming Medical Uni-versity, Kunming 650101, China; 2. Dept. of Medicine and Physiology, University of Toronto, Ontario M5S 1A8, Ca-nada; 3. Dept. of Plastic Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China） [Abstract] Objective The study is to observe the effect of nitric oxide（NO） donor and scavenger to the hypo- glossal motor nucleus（HMN） activity and explore the underlying mechanism. Methods Male adult anesthetized Wis-tar rats were anesthetized. The activity of genioglossus（GG）, diaphragma, blood pressure（BP） and respiratory rate（RR） were recorded when constant microdialysis perfusion of artificial cerebrospinal fluid（ACSF） to HMN as control, fol- lowed with diethylamine NONOate sodium salt hydrate（DEA）, a NO donor, and 2-（4-carboxyphenyl）-4,4,5,5-tetra- methylimidazoline-1-oxyl-3-oxide potassium salt（carboxy-PTIO）, a NO scavenger. Results Compared with ACSF, application of DEA and carboxy-PTIO at HMN increased and decreased the GG activity respectively and significantly（P0.05）, mainly respiratory-related activity. The tonic GG, diaphragma activity, BP and RR had not been affected statistically between 30-120 min when microdialysis perfusion of both DEA and carboxy-PTIO were delivered. Con-clusion Acting as an excitatory neurotransmitter of HMN, NO may con