ALA-PDT对兔耳增生性瘢痕抑制效应及作用机制初步探究.docVIP

ALA-PDT对兔耳增生性瘢痕抑制效应及作用机制初步探究[摘要]目的：观察ALA-PDT (5-氨基酮戊酸光动力疗法)对兔耳增生性瘢痕形成的抑制作用及对转化生长因子β1（TGF-β1）表达的影响。方法：在兔耳腹侧面做直径1cm大小全层皮肤缺损创面3个/侧，共60个。随机分为以下三组：高浓度ALA-PDT组(n=20)：20%ALA介导PDT治疗；低浓度ALA-PDT组(n=20):10%ALA介导PDT治疗;对照组（n=20）：不做治疗，待创面自然愈合。在创面形成术后的第6天、第13天分别进行ALA-PDT治疗，观察治疗组与对照组之间的瘢痕形成率、瘢痕增生指数、胶原纤维含量及TGF-β1表达情况的差异。结果：ALA-PDT治疗降低了瘢痕形成率，其中药物高浓度ALA-PDT组与对照组的差异有统计学意义（P＜0.01）；药物高浓度ALA-PDT组和药物低浓度ALA-PDT组的瘢痕增生指数、胶原纤维含量与对照组的差异有统计学意义（P＜0.05）；药物高浓度ALA-PDT组与对照组之间的TGF-β1的表达差异具有统计学显著性（P＜0.05）。结论：ALA-PDT对兔耳增生性瘢痕的形成有抑制作用，从而预防创伤后增生性瘢痕形成，该作用可能通过减少TGF-β1的产生而实现。 [关键词]光动力疗法；增生性瘢痕；胶原纤维；转化生长因子β1 [中图分类号]R619+.6 [文献标识码]A [文章编号]1008-6455（2011）01-0083-04 Preliminary study in the inhibitory effect and mechanism of ALA-PDT on the hypertrophic scar model in rabbit ears ZENG Wei-hui,WANG Qian-qian,QIN Jing-jing,GENG Song-mei,XU Lei,NIE Jian-jun (Department of Dermatology,Second Affiliated Hospital,Xi’an Jiaotong University,Xi’an 710004,Shaanxi,China Abstract:ObjectiveTo investigate the ability of ALA-PDT (5-Aminolaevulinic Acid-based Photodynamic Therapy)in reducing hypertrophic scar formation in a rabbit ear model and further to observe the expression of TGF-β1 (transforming growth factor-β1).MethodsThe acute dermal wound models on white rabbits’ ears were established. 60 scars were randomly divided into 3 groups: the high concentration ALA experimental group (n=20) received 20%ALA PDT treatment while the low one (n=20) received 10%ALA PDT treatment, and the controlled group (n=20) received no special treatment. The experimental groups received ALA-PDT treatment at two time points postwounding:days 6,13. Specimens were harvested from scars on postoperative 28 days and scar elevation index, fiber collagen contents were measured,TGF -β1 expression was tested by immunochemical staining.ResultsCompared with the controlled group, scar formation rate in high concentration ALA-PDT treatment group was reduced significantly (P 1.2.2 处理过程：剪大小约1.2cm的圆形医用棉，覆盖于创面，将配好的5-氨基酮戊酸药液浸湿医用棉，再用保鲜膜贴在