正常大鼠脊髓内微血管及TrkC分布的研究.pdfVIP

昆明 医学 院学报 20o8，(1)：43—45 CN 53—1O钠l／R Journal of Kunming Medical University 正常大鼠脊髓内微血管及TrkC分布的研究 黄纯海 一，庞江霞∞，王廷华∞ (1)中南大学湘雅医院神经外科，湖南长沙 410008；2)昆明医学院神经科学研究 所，云南昆明 650031) [摘要]目的 研究正常大鼠脊髓内微血管及TrkC分布情况及意义．方法 采用墨汁灌注及免疫组织化学 双标技术显示大鼠脊髓内微血管及TrkC分布，结果 脊髓微血管主要分布在灰质，而且在灰质中腹角微血管 密度大于背角，腹角、背角及白质三个部位之间的平均血管密度相比均有显著性差异 (P0．01)；在正常脊髓 中，TrkC免疫阳性反应物主要分布在腹角、腹角外侧、中央管背外侧及背角深部．结论 微血管及1 c主要 分布于脊髓腹角，以Ix板层最为显著，提示脊髓运动神经元的存活及其完成生理功能时亦需要大量的血供及 神经营养因子NT-3的支持． [关键词]脊髓；微血管；TrkC [中图分类号】Q426；R651．2 [文献标识码】A[文章编号2008)01—0043—03 Study on the Expression of TrkC and Distribution of Capillary Vessels in Normal Spinal Cord of Rats HUANG Chun—hai ，∞，PANG Jiang—xia∞，WANG Ting—hua∞ (1)Dept．ofNeurosurgery，Xiangya Hospital，Central South University，Changsha Hunan 410008； 2)Institute of Neuroscience，Kunming Medical University，Kunming Yunnan 650031，China) [Abstract]Objective To investigate the distribution of capillary vessel and expression of Trkc in spinal cord of rat．Methods In this study，double staining technique，Chinese ink perfusion and immunohistochem— istry ABC method．were used to investigate the distribution of capillary vessel and expression of TrkC in spinal cord of rat．Results Capillary vessels were mainly distributed in the grey matter of spinal cord，especially in ventral horn．The microvascular density was significantly different among ventral horn．dorsal horn and white matter of spinal cord(P0．01)．TrkC—like immunoreactivity was mainly distributed in neurons including venial horn， lateral ventral horn and dorsal—lateral central cana1．In addition。a few TrkC—like immunoreactivity was also found in the deep of dorsal horn．Conclusion The present results demonstrated that capillary vessels and TrkC mainly