纳米纤维家蚕丝蛋白软支架制备及性能 - 应用昆虫学报.pdfVIP

Chinese Journal of Applied Entomology 2015, 52(6): 15071512. DOI: 10.7679/j.issn.20951353.2015.182 纳米纤维家蚕丝蛋白软支架制备及性能* 1** 1 2 1*** 宁洪艳 韩宏岩 吕 强 许维岸 1. 215123 2. / 215123 摘 要 【目的】 【方法】 【结果】 Silk II 【结论】 关键词 Preparation and properties of nano-fiber, soft Bombyx mori silk protein scaffolds NING Hong-Yan 1** HAN Hong-Yan 1 LÜ Qiang2 XU Wei-An 1*** (1. School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China; 2. National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China) Abstract [Objectives] Regulating the nature of silk protein solution allows the control of the mechanical and structural properties of silk protein scaffolds suitable for the growth of endothelial cells, and solves the problem of engineering thick complex organs and tissues that require functional vasculature. [Methods] To study the effect of different silk protein scaffold properties nanofiber-assisted lyophilization was used to restrain separate lamella formation in silk scaffolds. In addition, the structure of silk was controlled by adjusting silk-water interactions, thereby obtaining different silk protein solutions, and through preparing scaffolds by lyophilization. [Results] Increasing silk-water interactions resulted in reduced silk II (β-sheet crystal) formation, which resulted in a decrease in scaffold formation. [Conclusion] Scaffolds with a suitab