己酮可可碱对大鼠脓毒症急性肺损伤p38MAPK活性的影响.PDF

己酮可可碱对大鼠脓毒症急性肺损伤p38MAPK 活性的影响 崔修德 1 ，封光 2 ，张稳稳 2 ，刘功俭 2*(1.河南省焦作市第二人民医院麻醉科，河南 焦作 454001 ；2.徐州医学院附属医院麻醉科， 江苏 徐州 221002) 摘要：目的 探讨己酮可可碱(pentoxifylline ，PTX)对腹腔感染致脓毒症急性肺损伤发挥肺保护作用与 p38MAPK 活化的 关系。方法 采用盲肠结扎穿孔致脓毒症模型，将大鼠随机分为Ⅰ组(Sham 组) 、Ⅱ组(脓毒症 CLP 组) 、Ⅲ组(脓毒症加西 黄著胶 CLP+V 组) 、Ⅳ组(脓毒症加生理盐水 CLP+N 组) 、Ⅴ组(脓毒症加 SB203580 CLP+SB 组) 、Ⅵ组(脓毒症加己酮可 可碱 CLP+PTX 组) ，其中Ⅲ组、Ⅳ组为溶媒对照组。用 Western Blot 检测假手术组，脓毒症 1，3 ，6 ，12，24 h 后 p38MAPK 的磷酸化，然后选择 1，6 ，24 h 分别检测应用 SB203580 或 PTX 后 p38MAPK 的表达，同时检测血浆 TNF-α、IL-6 的含 量并观察 24 h 内肺组织病理改变。结果 与假手术组比较，脓毒症组在各个时间点 p38MAPK 均有较强的表达，SB203580 或 PTX 预处理后各组的 p38MAPK 的磷酸化明显受到抑制，且与血浆 TNF-α、IL-6 的含量以及肺的病理切片变化一致。 结论 己酮可可碱可能是通过抑制 p38MAPK 的磷酸化抑制促炎因子的过度表达，发挥对脓毒症急性肺损伤的保护作用。 关键词：脓毒症；急性肺损伤；己酮可可碱；p38MAPK 中图分类号：R962.1 文献标志码：A 文章编号：1007-7693(2010)09-0767-05 Effect of Pentoxifylline on the Activation of p38 Mitogen Activated Protein Kinase in Rats with Septic Acute Lung Injury 1 2 2 2* CUI Xiude , FENG Guang , ZHANG Wenwen , LIU Gongjian (1.Department of Anesthesia, Jiaozuo Second People’s Hospital, Jiaozuo 454000, China; 2.Department of Anesthesia, Affliated Hospital of Xuzhou Medical College, Xuzhou 221002, China) ABSTRACT: OBJECTIVE To explore the effect of pentoxifylline (PTX) on the activation of p38MAPK in rats with septic acute lung injury induced by intra-abdominal infection. METHEDS SD rats were subjected to sepsis caused by cecal ligation and puncture(CLP), and animals were randomly divided into Ⅰ group(sham operation group), Ⅱ group(sepsis CLP group), Ⅲ group (sepsis+tragacanth CLP+V group), Ⅳ group (sepsis+NS CLP+N group), Ⅴ group (sepsis+SB203580 CLP+SB group), Ⅵ group (sepsis+PTX CLP+PTX group). p38MAPK phosphorylation were measured in 1, 3, 6, 12, 24 h, respectively. 1, 6, 24 h after pretreated with SB203580 or PTX, p38MA