基于响应面分析法对烟叶中果胶测定方法的优化 - 中国烟草科学.pdfVIP

2015-10365 Chinese Tobacco Science 85 1 2 2 2 2 2 2,3* 1. 650202 2. 650106 3. 650500) -Box-Benhnken pH pH 1.9 h56 ℃pH 3.8 R2=0.99999.2%~100.9%RSD n=63% Box-Behnken TS411 1007-5119201505-0085-05 DOI 10.13496/j.issn.1007-5119.2015.05.016 The Optimization of the Method of Tobacco Pectin Determination Based on the Response Surface Methodology 1 2 2 2 2 2 2,3* BAI Xiaoli , HOU Ying , ZHANG Lang , PAN Chunxiang , TAO Ziwei , LIU Jing , YANG Lei (1. Technology Centre, China tobacco Yunnan industrial Co., Ltd., Kunming 650202, China; 2. Testing Centre, Yunnan Comtestor Co., Ltd., Kunming 650106, China; 3. Faculty of Enviromental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China) Abstract: In order to optimize the method of determining tobacco pectin using enzymolysis-flow method, reaction temperature, enzymatic hydrolysis time and buffer pH were studied through the Box-Benhnken experimental design and response surface methodology. The results showed that reaction temperature had the greatest impact on the determination of pectin, followed by buffer pH, and the enzymatic hydrolysis time comes last. The optimal enzymatic hydrolysis conditions were pH 3.8, time 1.9 h and 56 ℃. Under this condition, the linear relationship of pectin was optimal (R2=0.999), with the recovery of pectin in tobacco ranged from 99.2% to 100.9%, and the RSD (n=6) was less than 3%. This suggested that the optimized enzymatic hydrolysis conditions used by the Box-behnken design and response surface analysis have good precision and accuracy and are suitable for testing l