百合转基因研究进展 - 中国农业科学院.pdfVIP

生物技术通报 BI OTECHN OLOG Y B ULLETI N 2005 5 1, 2 1* 2 1 1 尹丽蓉 谢丙炎 肖启明 杨宇红 葛红 1 2 ( , 100081; , 410128 ) : 从百合遗传转化受体的建立和百合转基因体系的建立两方 综述了建立百合的遗传转化体系的研 究情况, 后者包括抗生素的选用转化再生植株的筛选共培养时间农杆菌菌株的选择同时对近10 年来在百合 遗传转化方 所作的研究工作进行了综述, 包括转报告基因, 转抗病抗虫和抗病毒基因, 以及转化技术的研究 提出了转基因百合存在的问题, 并就其前景进行了展望 : 百合 遗传 受体 目的基因 转化技术 Advances of the Research on Transgenic Lilium 1, 2 1* 2 1 1 Yin Liro ng Xie Bingyan Xiao Qiming Yang Yuho ng Ge Hong ( 1 I nstit ute of vege table s and f low er s, Chine se A ca demy of A g ricultur al S cience s, Beij ing 100081; 2 H unan A gr icu lt ura l Univ er sity , Chang sha 410128 Abstract: T he author summari ed how t o establish g enetic transfor mation sy st ems of L ilium w ere com posed of establishing g enetic t ransformat ion acceptor systems and establishing tr ansg enic systems of some of lilium culti- v ars, the latter included choice antibiot ics and r egeneratio n plants, co-cultur ed time, opt imal A g r obacter ium Str ain and so o n. In recent few decades, a w ide r ang e of new carefully studying and new reports have been developed for va- r ious genetic t ransformation obj ects, r eports gene, resistant diseases, insect and t ransferred to v irus g ene , and tr ans- formation technology. Finally pr esented to exist questions and pr ospects about tr ansg enic lil ium . Key w ords: L ilium Genetic A cceptor T arget g ene T ransfor mation technology , , , 1957 Robb , , , , , , , 1. 1 再生植株途径 ,