半夏蛋白抗肿瘤活性组分的提取分离 - 中国中医药信息杂志.pdfVIP

2007年 1月第 14卷第 1期 中国中医药信息杂志 ·45· 半夏蛋白抗肿瘤活性组分的提取分离 付 芸,黄必胜,李 娟,田代志,陈科力 (湖北中医学院,湖北 武汉 430061) 摘要：目的 分离半夏蛋白的抗肿瘤活性组分,研究该半夏蛋白组分的抗肿瘤作用。方法 用凝胶柱分离纯化半 夏粗总蛋白,采用四氮唑蓝比色法(MTT 法)观察半夏蛋白对肝癌 Bel-7402 细胞增殖的抑制,应用流式细胞术(FCM)检 测其对细胞凋亡的影响。结果 半夏粗总蛋白中30%硫酸铵沉淀部分、60%硫酸铵沉淀部分对肿瘤抑制的量效关系不 明显,30%硫酸铵沉淀部分经分离后 0.05 mol/L 和 0.1 mol/L NaCl 的洗脱峰呈现较好的量效关系,且对 Bel-7402 细 胞的抑制作用与空白对照组有显著性差异。30%硫酸铵沉淀蛋白有促进 Bel-7402 细胞凋亡的作用。结论 半夏蛋白 中30%硫酸铵沉淀部分对Bel-7402细胞生长具有明显抑制作用及促进Bel-7402细胞凋亡的作用。 关键词：半夏；Bel-7402细胞；抗肿瘤蛋白组分；抗肿瘤活性测定 中图分类号：R284 文献标识码：A 文章编号：1005-5304(2007)01-0045-03 Extraction and Isolation of the Protein Groups with Anti-tumor Activity from Pinellia Ternata Rhizhome FU Yun, HUANG Bi-sheng, LI Juan, et al (Hubei College of TCM, Wuhan 430061, China) Abstract ：Objective To isolate the anti-tumor protein groups from Pinellia ternata rhizome and investigate the anti-tumor activity of these protein groups on Bel-7402 cell. Methods The total raw protein was isolated with sepharose column chromatography. Methyl-thiazolyl-tetrazolinm (MTT) was used to analyze the effect of Pinellia ternata Protein on inhibiting growth of tumor cells. Apoptosis was detected by flow cytometry (FCM). Results The 30% and 60% (NH ) SO deposition part of total proteins from Pinellia 4 2 4 ternata rhizome have no certain relationship between quantity and inhibitory action, but protein peak 3 eluted with 0.05 mol/L or 0.1 mol/L NaCl from 30% (NH ) SO deposition part showed a effect of 4 2 4 concentration