CMV感染监测在同种异基因造血干细胞移植中的应用.pdfVIP

Journal of Medical Forum Vo1．29 No．4 February 2008 · 7 · CMV感染监测在同种异基因 造血干细胞移植中的应用 蒋东霞，周 健，杨晓博 ，胡杰英，赵婷茹 河南省肿瘤研究所 郑州市 450008 捅要 目的 探讨不同方法CMV监测在同种异基因造血干细胞移植中应用的可行性。方法 应用荧光 定量PCR(FQ—PCR)和酶联免疫(ELISA)试剂盒分别检测l3名allo—HSCT受者、170份标本的血浆DNA负荷 量和血清IgM抗体。同时应用流式细胞术(FCM)检测94份标本白细胞0065抗原。结果 FQ—PCR、FCM、 ELISA检出率分别为：35．51％、30．85％、13．08％。FQ—PCR与FCM方法对CMV感染的阳性检出率差别无统 计学意义(P0．05)，ELISA的阳性检出率明显低于FQ—PCR和FCM，其差别具有统计学意义(P0．05)。 结论 FCM检测0065抗原和FQ—PCR检测DNA负荷量可用于allo—HSCT后CMV感染的早期诊断。 关键词 巨细胞病毒；荧光定量PCR；流式细胞术 中图分类号：R457．7 文献标识码：A 文章编号：1672-3422(2008)04-0007-04 Application of Human Cytomegalovirus Infection in Allogeneic Hematopoietic Stem Cell Transplantation JIANG Dongxia，ZHOUJian，YANG Xiaobo，et al Henan Institute ofTumor，Zhengzhou 450008，China ABSTRACT Objective To explore the application possibility of different detection methods for eytomegalovirus(CMV)in allogeneic hematopoietic stem cell transplantation(allo—HSCT)． Methods The plasma DNA loading and serum level of IgM antibody against CMV in 170 clinical specimens from 1 3 allo—HSCT patients were detected by real—time FQ—PCR and ELISA respective— ly． e pp65 antigen in 94 peripheral blood leukocyte samples was measured by FCM．Results e positive rates of FQ—PCR，FCM and ELISA were 35．51％．30．85％and 13．08％respectively．There was no statistical significant difference between the positive rate of FQ—PCR and FCM to the CVM in— feetion amount(P0．05)．The positive rate of IgM anti—body detected by ELISA was obviously lower than that of DNA quantized by FQ—PCR and pp65 antigen detected by FCM，but the difference between them showed statistical significance(P0．05)．Conclusion FQ—PCR and FCM are relia- ble methods for monitoring recipient reactive