局部晚期宫颈鳞癌流式细胞术分析与放射治疗预后的关系.pdfVIP

·940· 肿瘤防治研究2007年第34卷第12期 局部晚期宫颈鳞癌流式细胞术分析与 放射治疗预后的关系 张孟贤，韩 娜，于世英 Prognostic Significance of Flow-cytometric DNA Anaysis in Locally Advanced Cervical Sq／ us Carcinoma ZHANG Meng—xian．HAN Na．YU Shi—ying Department of Oncology，丁’0 Hospital，rongji Medical College，Huazhong University of Science and Technology。Wuhan 43003(I．C矗ina Abstract：Objective To determine the utility of DNA flow cytometry as a prognostic indicator for risk of recurrence and survival in patients with locally advanced squamous cervical carcinoma．Methods DNA flow cytometry was performed tO determine ploidy，S phase fraction(SPF)，and proliferative index(PI)in 68 women with stageⅢB squamous carcinomas of the cervix treated by radiotherapy(RT)．Sixty-eight tissue samples were obtained by punch biopsy from cervical primary tumor before RT．Flow-cytometric results，along with other known prognostic variables for risk for recurrent disease and survival，were ana— lyzed using COX regression proportional hazards model，and survival curves were generated by the Kaplan Meier method．Results Of 68 samples，DNA ploidy patterns were 36(52．9 )diploid and 32(47．1 ) aneuploid．The mean values of SPF and P1 were 7．49±2．9 1 and 1 2．89±3．75 respectively．Thirty of 68 patients(44．1 )experienced recurrence during the follow-up．Aneuploid patients had lower 5-year re— current free survival than those with diploid pattern(35．2 VS．52．7 ；P％0．05)and high SPF group had 1ower 5-year recurrent free survival than low SPF group as well(25．3 VS．56．6 ；P％0．05)．Mul— tivariate analysis confirmed that only SPF and tumor size were the significant factor for 5-year recurrent free surva1(PO．05)．Conclusion DNA ploidy and PI are not independent prognostic factors for tumor recurrence；however，a high SPF warrants further investigation as a potential prognostic indicator for risk of recurrent disease in patients with squamous carcinoma of the uterine cervim