增殖细胞核抗原、表皮生长因子受体和B淋巴细胞瘤基因在非小细胞肺癌中的表达及临床意义.pdfVIP

2008年1月第3卷第1期 China medicine，January 2oo8，Vo1．3．No．1 ． 论著 ． 增殖细胞核抗原、表皮生长因子受体和 B淋巴细胞瘤基因在非小细胞肺癌中 的表达及临床意义 张绘宇 马宁芳 李书华 张雅洁 【摘要】 目的 研究增殖细胞核抗原(PCNA)、表皮生长因子受体(EGFR)和B淋巴细胞瘤基因 (BCL-2)3种基因蛋白与非小细胞肺癌(NSCLC)临床病理特征的关系。方法 应用S．P免疫组化法检测44 倒手术切除NSCLC标本中3种基因蛋白的表达。结果 44例标本中37例(84．09％)PCNA过度表达，30例 (68．18％)EGFR过度表达，l9例(43．18％)BCL-2过度表达。PCNA、EGFR及BCL-2阳性表达率在NSCLC 淋巴结有元转移患者间均有统计学意义(P0．01或P0．05)。PCNA和EGFR阳性表达率与患者生存时 间长短组问有统计学意义(P0．01或P0．05)。但是3种蛋白阳性表达率均与肿瘤大小、分化程度无相 关性(P0．05)。结论 PCNA、EGFR和BCL-2基因过度表达可能与NSCLC的转移、预后有关。 【关键词】 非小细胞肺癌； 免疫组化； 增殖细胞核抗原； 表皮生长因子受体； B淋巴细胞瘤基因 【中图分类号】R 734．2【文献标识码】A 【文章编号】1673-4777(2008)01-0014-03 E羽，r 0n and significance of proliferating cell nuclear antigen，epidermal growth factor receptor and B cell lymphoma-2 gene in non—small cell lung cancer Zhang Huiyu，Ma Ningfang，Li Shuhua，Zhang Yafie． Department ofPathology，Guangzhou Medical College(Guangzhou 510182，China) 【Abstract] Objective To explore the relationship between the expression of protein of proliferating cell nuclear antigen(PCNA)，Epidermal Growth Factor Receptor(EGFR)，BCL-2 and pathological characteristics of non．small cell lung cancer(NSCI )．Methods Protein levels of PCNA．EGFR and BCL-2 were detected in 44 NSCLC samples by immunohistochemistry．Results PCNA was positive in 37 cases(84．09％)．EGFR was 68． 18％ and BCL-2 Was 43．】8％ respectively．The expressions of PCNA．EGFR and BCL-2 protein between tl1e group oflymph node metastasis and the group of non—lymph node metastasis were significant different(P0．05)．The expressions of PCNA and the EGFR showed significant relation with the survival time(P0．05)．But expressions ofthe thlee proteins were not significantly related with the size and differentiation of the tumor(P0．05)． Conclusion Overexpression of PCNA，EGFR and BCL-2 may play an impo~ant role in the oncogenesis an