壳寡糖诱导对烟草体内TMV—CP基因表达的抑制作用.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 37(6)：637-641(2007) 壳寡糖诱导对烟草体内TMV—CP基因 表达的抑制作用 商文静 ，吴云锋 ，商鸿生 ，赵小明。，杜昱光。 ( 西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室，杨凌712100；。中国科学院大连化学物理研究所，大连116023) 摘要：采用实时荧光定量PCR方法，研究了壳寡糖诱导对烟草体内TMV—cP基因表达量的影响。结果表明，壳寡糖诱导 后烟草表达了对TMV侵染的系统获得抗病性，植株显症推迟4—7 d，症状减轻，平均严重度比对照降低了82．9％。同时烟 草叶片中CP基因的表达量显著降低。壳寡糖诱导处理的植株在接种病毒时(0 h)，CP基因的表达量(拷贝数／2 tLL)为 0．918，接种后168 h增加到167．730。而在不诱导对照植株内，CP基因的表达量在0 h为l_218，接种后168 h猛增到 648．623。换言之，不处理对照的表达量在此期间增长了532．53倍，而壳寡糖诱导处理植株中仅增长了182．71倍。试验证 明壳寡糖诱导的系统获得抗病性强烈抑制了烟草体内TMV—CP基因的表达。 关键词：壳寡糖；系统获得抗病性；TMV—CP基因；实时荧光定量PCR Inhibitory effect to TMV—CP gene expression in tobacco induced by chito—oligosac— charides SHANG Wen-jing ，wU Yun—feng ，SHANG Hong．sheng ，ZHAO Xiao—ming。，DU Yu—guang ( College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture，Northwest AF University，Yangling 712100，China； Dalian Institute of Chemical Physics，Chinese Academy of Sciences，Dalian 116023， China) Abstract：The inhibitory effect to TMV—CP gene expression in tobacco(Nicotiana tabacum)seedlings in— duced by chito—oligosaccharides was examined with real—time quantitative RT—PCR (QPCR)．The results showed that high level systemic acquired resistance(SAR)was expressed in tobacco plants treated with solu— tion of chito—oligosaccharides at the concentration of 50~g／mL，the latent period was 4—7 d delayed and the average disease severity was 82．9％lowered．Meanwhile，the expression quantity(copies／2 L)of CP gene in treated plants was reduced remarkably，it was 0．918 at 0 h(the time of virus inoculation)and 167．730 at 168 h after inoculation．But，in untreated plants the expression quantity was 1．218 at 0 h，and 648．623 at 168 h．During this time span that increased 532．53 times in untreated plants，and only 182．71 times in treated plants．It was concluded that the expression of TMV—CP gene in tobacco was strongly suppressed by SAR in— duced