宁夏葡萄酒中白藜芦醇含量的高效液相色谱快速测定_冯愈钦.pdfVIP

2017 年3 月 食品研究与开发 第38 卷第5 期 Food Research And Development 标准与检测 156 DOI ：10.3969/j.issn.1005-6521.2017.05.033 宁夏葡萄酒中白藜芦醇含量的高效液相色谱 快速测定 * 冯愈钦，郭红艳，刘贵珊，何建国，王松磊，贺晓光 （宁夏大学农学院，宁夏银川750021） 摘 要：建立高效液相色谱法测定葡萄酒中白藜芦醇的方法，考察直接进样法的检测波长、洗脱强度、流动相pH 值、柱 温、样品pH 值等因素对酒样分离的影响，建立HPLC 检测葡萄酒中白藜芦醇的方法，相关系数r 为0.999 9 ，线性范 围为1 mg/L ~20 mg/L，精密度、稳定性、回收率试验的RSD 分别为1.16 %、2.9 %和1.25 %，酒样检测发现白藜芦醇的含 量随酒的年份和品种变化，品种间比较结果为：蛇龙珠最优，梅鹿辄、赤霞珠、霞多丽次之，贵人香最低，其中蛇龙珠含量 高达6 mg/L。 关键词：白藜芦醇；高效液相色谱；回收率；葡萄酒 Rapid Determination of Resveratrol in Ningxia Wine by High Performance Liquid Chromatography * FENG Yu-qin ，GUO Hong-yan，LIU Gui-shan ，HE Jian-guo ，WANG Song-lei ，HE Xiao-guang （Agricultural College ，Ningxia University ，Yinchuan 750021 ，Ningxia ，China） Abstract ：The effects of detection wavelength ，solvent strength ，mobile phase pH ，column temperature ，sample pH and other factors on the separation of wines were examined.A direct injection HPLC method for determina－ tion of resveratrol in wine was established. The correlation coefficient r was 0.999 9 ，linear range was 1 mg /L- 20 mg/L ，RSD of precision experiment ，stability experiment and recovery experiment were 1.16 % ，2.9 % and 1.25 % respectively.It was found that resveratrol content of wines varied with years and varieties ，its changing law was ：Cabernet Gernischt was the best chioce ，Merlot and Cabernet Sauvignon and Chardonnay took second ， the last was Riesling. Resveratrol content in Cabernet Gernischt was up to 6 mg /L. Key words ：resveratrol ；HPLC；rate of recovery ；wine 世界酿酒葡萄种植的地带是北纬30 ℃～50 ℃和