气相色谱外标法测定环十五内酯.pdfVIP

※分析检测 食品科学 2009, Vol. 30, No. 10 159 气相色谱外标法测定环十五内酯 赖 芳，刘雄民* ，李伟光，马 丽 (广西大学化学化工学院，广西南宁 530004) 摘 要：进行蒜头果油合成环十五内酯的定量分析方法研究，用气相色谱外标法建立环十五内酯的定量分析方法， 以环十五内醋的峰面积(A)对应的浓度(C)进行线性回归分析，得到通过原点的回归方程为：A=223 8.7C(r=0.9993) ， 可以利用单点校正对标准曲线进行校正，回收率为95. 11% ～103 .3% ，并利用该法跟踪测定了蒜头果油合成环十五 内酯产物的含量。 关键词：环十五内酯；气相色谱法；外标法 Determination of Cyclopentadecanolide Synthesized from Malania oleif era Chun et S.K. Lee Kernel Oil by Gas Chromatography with External Standard Method LAI Fang ，LIU Xiong-min * ，LI Wei-guang ，MA Li (School of Chemistry and Chemical Engineering ，Guangxi University, Nanning 530004, China) Abstract ：Gas chrom atography (GC) w ith area-extern al stan dard m eth od was applied to qu antitative an aly sis of cyclopentadecanolide in its synthesis from Malania oleif era Chun et S.K. Lee kernel oil. A linear regression equation was obtained, which was A = 2238.7C (r = 0.9993), where A was the GC peak area, and C was the cyclopentadecanolide concentration, and the standard curve was validated by single-point external bracketing standard. The recoveries of cyclopentadecanolide in 3.578 mg of sample at the spiked amounts of 1.977, 3.954 and 5.931 mg were 95.11% to 103.3%, the RSDs were 1.51% to 2.19% (n = 3). The determination results of cyclopentadecanolide content in four prepared sample solutions were 2.14 to 4.52 mg/ml, and the RSDs were 0.66% to 2.36% (n = 3). Key words ：cyclopentadecanolide ；gas chromatography ；area-external standard method 中图分类号：O657.7 1 文献标识码：A 文章编号：1002-6630(2009) 10-0 159-03 环十五内酯为无色针状结晶，具有强烈的天然香 催化剂作用下关环合成大环内酯。 气，并有龙涎香韵，是高价值的麝香类原料成分，用 蒜