储藏微环境下小麦细胞线粒体超微结构和抗氧化酶活性变化研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.3 储藏微环境下小麦细胞线粒体超微结构和 抗氧化酶活性变化研究 张丽丽，王若兰，刘莉，穆垚 （河南工业大学粮油食品学院，河南郑州 450001） - 摘要：利用透射电镜观察人工模拟储藏微环境下小麦细胞线粒体超微结构，并同时测定线粒体中的 O2 ·含量、COX 活性，以及 种子活力和抗氧化酶（SOD、CAT、POD）的活性，研究在不同储藏微环境（A、B、C、D）下小麦线粒体超微结构和抗氧化酶活性 的变化规律，从微观角度探讨其与小麦衰老的关系。研究表明：随储藏时间延长和温湿度增高，线粒体数目减少，其超微结构的损伤 劣变不断加剧。主要表现为：外膜变形并出现破损溶解，内膜的脊减少，脊的双层膜结构逐渐模糊不清，线粒体内部降解，内腔出现 - 空泡化等；与此同时，O2 ·生成速率不断增大，而 COX 活性和种子活力不断下降。D 条件下三者变化最显著（P0.05）；抗氧化酶的 活性在高温高湿（C 和 D）条件下都经历了先增大后下降的过程，D 条件下下降最明显，降幅分别达到 36%、63%、57%，显著高于 低温低湿（A 和 B）条件（P0.05）。 关键词：小麦；储藏微环境；线粒体超微结构；抗氧化酶 文章篇号：1673-9078(2014)3-81-86 Variation of Mitochondrial Ultrastructure and Antioxidative Enzymatic activity in Wheat Cell under Micro-environmental Conditions ZHANG Li-li, WANG Ruo-lan, LIU Li, MU Yao (College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China) Abstract: The mitochondrial ultrastructure of wheat cell were investigated by transmission electron microscope under different simulated - micro-environmental conditions. Simultaneously, mitochondrial O2 ·, COX activity, seed vitality and the activity of antioxidative enzymes (SOD, CAT and POD) were measured, which were used to discuss the relationship between them and wheat aging at the micro perspective. The research showed that with the extension of storage time and increasing of temper