_德氏乳杆菌发酵生产乳酸工艺条件优化.pdfVIP

化 工 进 展 ·1332· CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2011 年第 30 卷第 6 期 研究开发 德氏乳杆菌发酵生产乳酸工艺条件优化 刘 鹏 1，贾晓强 1，2，杨春燕 1，闻建平 1，2，财音青格乐 1，2 （1 天津大学化工学院，天津 300072；2 天津大学系统生物工程教育部重点实验室，天津 300072） 摘 要：本实验在选育高产菌株及优化培养基的基础上，通过探索德氏乳杆菌的发酵工艺提高其乳酸产量。用 初始菌株在基础发酵培养基上进行乳酸（DL-乳酸）发酵，其中 L-乳酸的产量为 18.5 g/L，对葡萄糖的转化率为 50.27%。对原始菌株进行了激光诱变，选育出一株高产菌株 DBLB28，对其培养基进行了进一步优化。先用正 交实验设计法选出了主要的影响因子，然后再利用响应面法对所选因子进行优化，最优的条件为：葡萄糖浓度 68.6 g/L；牛肉膏浓度 22.4 g/L；蛋白胨浓度 3.2 g/L，从回归方程预计乳酸的最大产量为 29.0 g/L。在以上优化条 件下进行发酵实验，测得乳酸产量为 29.4 g/L，与预计值吻合较好。 关键词：德氏乳杆菌；乳酸发酵；正交实验法；响应面分析法 中图分类号：TQ 921+.1 文献标志码：A 文章编号：1000–6613（2011）06–1332–09 Optimization of fermentation conditions for production of lactic acid by Lactobacillus delbrueckii LIU Peng1，JIA Xiaoqiang1，2，YANG Chunyan1，WEN Jianping1，2，CAIYIN Qinggele 1，2 （1Department of Biochemical Engineering，School of Chemical Engineering and Technology，Tianjin University， Tianjin 300072，China；2 Key Laboratory of Systems Bioengineering， Tianjin University，Tianjin 300072，China） Abstract：This experimental work was primarily on the fermentation techniques of lactic acid，trying to enhance the production of lactic acid by mutation of the strain and optimization of the culture medium. The final concentration of L-lactic acid in the broth was 18.5 g/L using the basic medium with the original strain，and the conversion rate based on glucose was 50.27%. A high-yield strain was screened out by laser mutation from original strain，which was named DBLB28. The culture medium was optimized after screening out the high-yield strain. Several factors that had important effects