内毒素血症Wistar大鼠血清TNF—α与心肌细胞凋亡的关系.docVIP

内毒素血症Wistar大鼠血清TNF—α与心肌细胞凋亡的关系 　　[摘要] 目的 研究内毒素血症Wistar大鼠血清TNF-α与心肌细胞凋亡的关系及EPO对其的影响。 方法 将180只Wistar大鼠随机分为对照组、脂多糖LPS组及EPO处理组，分别在处理后2h、 4h、 6h、 12h、 24h断头取血并取其心脏。采用ELISA法测定血清TNF-α，用TUNEL法检测原位凋亡。 结果 ①LPS组TNF-α浓度在2h时达到最大值，且明显高于对照组（P0.05）。LPS+EPO组TNF-α浓度在2 h时达到最大值，且明显低于LPS组（P0.05），随后开始下降，在6h时降到正常值；②LPS组心肌细胞凋亡指数在2h时达到最大值，且明显高于对照组（P0.05），随后开始下降，并在4h、6h、12h、24h时均明显高于对照组（P0.05）。LPS+EPO组心肌细胞凋亡指数在2h时达到最大值，且明显低于LPS组（P0.05），随后开始下降，并在4 h、6 h、12 h、24 h时均明显低于LPS组（P0.05）；③心肌细胞凋亡指数与血清TNF-α存在明显的直线正相关性。结论 血清TNF-α参与了内毒素血症Wistar大鼠的心肌损伤的病理过程，与心肌细胞凋亡呈正相关性，EPO通过抑制TNF-α的产生而延缓心肌细胞凋亡，对内毒素血症Wistar大鼠的心肌具有保护作用。 　　[关键词] 内毒素血症；TNF-α；心肌细胞凋亡指数；EPO 　　[中图分类号] R541 [文献标识码] A [文章编号] 1673-9701（2014）02-0001-03 　　A study on the relationship between endotoxemia serum TNF-α of Wistar rats and apoptosis of myocardial cell 　　SUN Wei WANG Xiaoping JIN Wenyang 　　Emergency Department， Taizhou Hospital， Enze Medical Center of Taizhou in Zhejiang province，Taizhou 317000，China 　　[Abstract] Objective To study the relationship of rat serum TNF-α of endotoxin blood disease and the apoptosis of myocardial cells and EPO’s effects on it. Methods 180 Wistar rats were randomly divided into the control group， lipopolysaccharide LPS group and EPO treatment group. All the rats were respectively beheaded 2h， 4h， 6h， 12h and 24h after treatment for their blood samples and their levers were also taken out. Their TNF-α were tested by way of ELISA， and their in situ apoptosis were tested through the method of TUNEL. Results ①The TNF-α concentration of the LPS group reached its highest level in 2 hours， and it was obviously higher than that of the control group（P0.05）. The TNF-α concentration of LPS+EPO group reached its peak in 2 hours， and it was obviously lower than that of the LPS group （P 　　[Key words] Dndotoxin blood disease； TNF-α； Apoptosis index of myocardial cells； EPO 　　近年来研究证实，机体在遭遇感染时首先产生的、起核心作用的炎症介质是肿瘤坏死因子（tumor necrosis factor，TNF），尤其是TNF-α。脂多糖（LPS）是引起感染性休克机体损伤的主要物质，可对机体形成广泛的影响及损伤，并引起心率减慢、心肌收缩力下降，严重影响心功能[1]