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尾巨桉DH32-29号的组织培养及快速繁殖研究 Study on Tissue Culture and Rapid Propagation for Fast Growing Eucalyptus of Eucalyptus urophy × Eucalyptus grandisDH32-29 尾巨桉DH32-29号的组织培养及快速繁殖研究 摘要 杂交组合尾巨桉DH32-29无性系，是中南几省桉树丰产林基地主要的种植品系。本试验主要通过组织培养手段，以MS和改良MS为基本培养基，利用6-BA、IBA、NAA等生长调节物质和GGR6号进行不同浓度及比例的组合，对以尾巨桉DH32-29的无菌苗为材料进行了继代增殖和生根培养实验，同时对尾巨桉DH32-29的外植体灭菌、炼苗和移栽进行了初步研究。实验结果表明消毒茎段最好的组合为：茎段尖端0.1%升汞4分钟，此时的污染率为45%，成活率为35%，茎段中段0.1%升汞6分钟，此时的污染率为60%，成活率为30%。经实验发现尾巨桉DH32-29组培苗在MS + 0.5mg/L 6–BA + 0.1mg/L NAA培养基进行继代增殖，增殖率较高，平均每株增值数为5.0。在1/2MS + 0.5 mg/L NAA + 0.1 mg/L IBA + 0.6mg/L GGR6号培养基上的幼苗根生长率达100%且生根较快，根比较健壮数目较多,平均每株生根数达到4.5根。由组培苗进行的移栽，经炼苗和用甲托喷洒处理成活率可达84%。 关键词：尾巨桉，组织培养，快速繁殖，继代增殖 Study on Tissue Culture and Rapid Propagation for Fast Growing Eucalyptus of Eucalyptus urophy × Eucalyptus grandisDH32-29 Biological Sciences 06 An jia-chan Supervisor Huang Zhao-Yu Abstract The clone of E.urophylla×E.grandis DH32-29 is the main planter strain in south central several province tuart bumper crop forest base.This experiment mainly was accomplished by tissue culture, with MS and modified MS as basic medium,used combinations of different concentrations and proportion of 6-BA,IBA,NAA,other growth regulators and GGR6, hardening and transplanting a preliminary study,right to sterile materials of E.urophylla×E.grandis DH32-29 were subcultured for the proliferation and rooting experiments,and explant sterilization of the E.urophylla×E.grandis DH32-29 The results show that the best combination of sterilization for the stem is: stem section 4 minutes, Mercuric chloride 0.1% tip, the rate of pollution is 45%, the survival rate is 35%, stem tip section for: 6 minutes in the middle Mercuric chloride 0.1%, the survival rate is 60%, pollutionis 30%. The experiment found that E.urophylla×E.grandis conduct following the generation of proliferation, multiplication rates and the average number of 3.5,in the medium,which is composited of E.urophylla×E.grandis DH32-29 in MS + 0.5 mg/L 6-BA + 0.1 mg/L NAA .But the s