Metabolic efficiency in yeast Saccharomyces cerevisiae in relation to temperature dependent growth and biomass yield》.pdfVIP

Journal of Thermal Biology 52 (2015) 117 –129 Contents lists available at ScienceDirect Journal of Thermal Biology journal homepage: /locate/jtherbio Metabolic efficiency in yeast Saccharomyces cerevisiae in relation to temperature dependent growth and biomass yield Maksim Zakhartsev a,b,c,n, Xuelian Yang b,d, Matthias Reuss b, Hans Otto Pörtner a a Alfred Wegener Institute for Marine and Polar Research (AWI), Bremerhaven, Germany b Institute of Biochemical Engineering (IBVT), University of Stuttgart, Stuttgart, Germany c Institute of Pharmacy and Molecular Biotechnology (IPMB), University of Heidelberg, Germany d Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology Business University, Beijing, China a r t i c l e i n f o a b s t r a c t Article history: Canonized view on temperature effects on growth rate of microorganisms is based on assumption of Received 26 January 2015 protein denaturation, which is not confirmed experimentally so far. We develop an alternative concept, Received in revised form which is based on view that limits of thermal tolerance are based on imbalance of cellular energy al- 29 May 2015 location. Therefore, we investigated growth suppression of yeast Saccharomyces cerevisiae in the su- Accepted 29 May 2015 praoptimal temperature range (30–40 °C), i.e. above optimal temperature (T