Label-free, Ultrasensitive and Electrochemical Immunosensing Platform for microRNA Detection Using Anti-DNARNA Hybrid Antibody and Enzymatic Signal Amplification》.pdfVIP

Electrochimica Acta 165 (2015) 130–135 Contents lists available at ScienceDirect Electrochimica Acta journal homepa ge: www.elsev /locate/electacta Label-free, Ultrasensitive and Electrochemical Immunosensing Platform for microRNA Detection Using Anti-DNA:RNA Hybrid Antibody and Enzymatic Signal Amplification Mo Wang 1, Bingchen Li 1, Qing Zhou, Huanshun Yin, Yunlei Zhou *, Shiyun Ai * College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, PR China A R T I C L E I N F O A B S T R A C T Article history: In this work, we proposed a selective and sensitive biosensor for microRNA detection based on the high Received 12 December 2014 specificity and affinity of anti-DNA:RNA hybrids antibody (S9.6 antibody) and alkaline phosphatase Received in revised form 2 March 2015 catalytic signal amplification. Briefly, after the hybridization of probe DNA and the target microRNA, the Accepted 2 March 2015 S9.6 antibody can be captured on the electrode surface through antigen-antibody immunoreaction. Then, Available online 5 March 2015 alkaline phosphatase labeled goat anti-mouse IgG (ALP-IgG) was further captured on the electrode surface through the specific recognition effect between the primary antibody and the secondary Keywords: antibody. Finally, ALP catalyzed the hydrolysis reaction of p-nitrophenyl phosphate to gen