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Isoforms of rat apolipoprotein A-I isolated from the lipoproteins of hepatic golgi apparatus and plasma》.pdf
Atherosclerosis, 56 (1985) 189-198 189 Elsevier Scientific Publishers Ireland, Ltd. ATH 03632 Isoforms of Rat Apolipoprotein A-I Isolated from the Lipoproteins of Hepatic Golgi Apparatus and Plasma P. Tarugi, M. Ghisellini, M. Pecorari, N. Brugni and S. Calandra * Isrituto di Patologia Generale, Unwersith di Modena, Via Campi 287, 41100 Modena (Iraly) (Received 5 July, 1984) (Revised, received 12 October, 1984; 17 January, 1985) (Accepted 21 January, 1985) Summary We compared apo A-I isolated from the lipoproteins of the Golgi apparatus of rat liver with apo A-I found in plasma lipoproteins. Golgi apo A-I consists of 3 main isoforms with a molecular weight of approximately 28 000 and isoelectric points (PI) of 5.97, 5.88 and 5.76, respectively. Plasma apo A-I consists of 4 major and 3 minor isoforms with a molecular weight of 27 000. The pI of the major isoforms (numbered 4-7) is 5.88, 5.80, 5.70 and 5.60, respectively. In order to investigate which of the plasma isoforms derived directly from Golgi apo A-I, [35S]methionine was injected into the portal vein and Golgi and plasma apo A-I were isolated shortly thereafter. While all Golgi isoforms were labelled only 3 isoforms of plasma apo A-I (namely isofo
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