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Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper》.pdf
Fish Shellfish Immunology 43 (2015) 396e404 Contents lists available at ScienceDirect Fish Shellfish Immunology journal homepage: www.elsevier.c om/locate/fsi Short communication Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper Jingguang Wei a, b, Pin Gao c, Ping Zhang d, Minglan Guo a, b, Meng Xu c, Shina Wei a, b, Yang Yan a, b, Qiwei Qin a, b, * a Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 51030 1, PR China b Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China c State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources, College of Marine Science, Hainan University, Haikou 570228, PR China d Teaching Center of Biology Experiment, School of Life Sciences, Sun Yat-sen University, 135 West Xingang Road, Guangzhou 510275, PR China a r t i c l e i n f o a b s t r a c t Article history: Apolipoproteins, synthesized mainly in liver and intestine and bounded to lipids, play important roles in Received 22 October 2014 lipid transport and uptake through the circulation system. In this study, an apolipoprotein A-I gene Received in revised form homologue was cloned from orange-spotted grouper Epinephelus coioides (designed as Ec-ApoA-I) by 5 January 2015 rapid amplification of cDNA ends (RACE) PCR. The full-length c
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