Isolation of a putative probiotic strain S12 and its effect on growth performance, non-specific immunity and disease-resistance of white shrimp, Litopenaeus vannamei》.pdfVIP

Fish Shellfish Immunology 41 (2014) 300e307 Contents lists available at ScienceDirect Fish Shellfish Immunology journal homepage: www.elsevier.c om/locate/fsi Full length article Isolation of a putative probiotic strain S12 and its effect on growth performance, non-specific immunity and disease-resistance of white shrimp, Litopenaeus vannamei Hongyu Liu a, Zheng Li b, Beiping Tan a, *, Ye Lao b, Zhiyong Duan b, Wuwei Sun a, Xiaohui Dong a a Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang 524025, PR China b Kemin Industries (Zhuhai) Co. Ltd, Zhuhai 519110, PR China a r t i c l e i n f o a b s t r a c t Article history: The common pathogens in aquaculture are very different from those in terrestrial animals. The objective Received 17 April 2014 of this study was to isolate probiotic strain (s) from the digestive tract of healthy white shrimp Litope- Received in revised form naeus vannamei which was effective against aquatic animal pathogens. The putative probiotic strain S12 22 August 2014 was identified as Bacillus subtilis based on the morphological and biochemical properties and 16S rDNA Accepted 25 August 2014 gene sequencing. The L. vannamei were fed with five different diets: control (basal diet with no probiotics Available online 16 September 2014 or antibiotics), antibiotic control (basal diet supplemented with 0.3% florfenicol), basal diet supple-