Isolation of a cDNA clone for chick intestinal apolipoprotein AI (Apo-AI) and its use for detecting apo-AI mRNA expression in several chick tissues》.pdfVIP

Gene, 42 (1986) 209-214 209 Elsevier GENE 1566 Isolation of a cDNA clone for chick intestinal apolipoprotein AI (APO-AI) and its use for detecting upo-AI mRNA expression in several chick tissues (Recombinant DNA; plasmid expression vectors; antibody screening of cDNA library; nucleic acids hybridiza- tion ; tissue-specific gene expression) Stefano Ferraria*, Enoe Drusiania, Sebastian0 Calandrab and Patrizia Tarugi b “ Istitutodi Chimica Bioiogka, Tel. jO59/360045, and b Istitutodi Patologia Generale, Tel. (059/360044, UIriversitcidi Modem. Via Campi 287, 41100 ~~dena ~~ta~~~] (Keceived August i8th, 1985) (Revision received January lOth, 1986) (Accepted January 14th, 1986) SUMMARY Three cDNA clones for chick apolipoprotein AI (APO-AI), the major protein component of plasma high-den- sity lipoproteins, have been isolated. The identity of the clones has been established first by screening a cDNA library in the pEX1 expression vector with anti-Apo-AI antibodies, second by Western blot analysis of the proteins expressed by positive clones. The use of the clone containing the largest, presumably full-size, cDNA insert (qu5C12) in molecular hyb~dization experiments confirms that upo-AI mRNA is expressed mainly in chick small intestine and liver. Furthermore, we provide evidence that brain, heart and skeletal muscle also synthesize significant amounts of ape-AI mRNA. The Southe