高效液相色谱法分离和测定甜菊甙.pdfVIP

Reversed-PhaseLiquidChromatography ofSome mixtureofzinc-porphyrinscontainingdifferent PorphyrinsandMetalloporphyrinsHuangJi-i, numbersofp-chlorophenylandphenylgroupsat WangYan-hui,LuWi zhen,ang ui-bin, themeso-position(B). lumn cked th品 DalianInstiluleofChemicalPhysics,cademia YWG-C18(3-5)(5 50mm)wasusedandthe Sinica mobilephasewasmethanolcontaining5% H2O. TheRP-HPLCsystem hasbeenchosenfor A goodseparationbetween SamplesAandB separatingsomesymmetricallysubstitutedporph- wasobtained,hydroxyphenyl-,β trime-中 yrinsandtheirmetalloporphyrins,uchso,C,6 thoxyphenyl-porphyrin, -dihydroxyphenyl-, Znmeso-tetraphenylporphyrin;i,C,Znmeo-得煤 -dimethoxyphenyl-porphrin, dihydroxy- tetra(4-pyridyl)porphyrin;o eso-tetra m- dhenyl-, -dimethoxyphenyl-porphyrin and methylphenyl)porphyrin, o-tetra(p 试在 meso-tetra(p-methoxyphenyl)porphrininsam- methoxyphenyl)porphyrin,tc.Tecromatogra-S pleAwereidentifiedbyusingtwoauthentic phicpeakswereidentifiedbyfastscanningin samples.or ample,tetreemincroma-3 thevisibleregion.he eparation f everal tographicpeakswerecollectedonthesamecolumn unsymmetricallysubstitutedtetraarylporphyrins andconcentratedtothethreecrystalsinmilli- andmetalloporphyrins,uch s ixtrue f gramlevel.hen hey ere easured yS, porphyrinscontaining different numbers of NMR,-Vis.Th α?- orophenyl-,β?,γ s p-hydroxyphenylandp-methoxyphenylgroups triphenel-Zinc-porphyrinandmesotetra(p-chlo- atthe meso-position (Sample A) and a rophenyl)-Zinc-porphyrinwereidentified. 高效液相色谱法分离和测定甜菊甙 彭启华 樊绍文 王文龙 温（州市工业科学研究所） 提〔要〕本文研究了应用反相高效液相色谱法分离甜菊糖甙，对其中主要成分甜菊甙进行了 鉴定和定量测定。分析条件如下：柱，YWG-C18H37;流动相，甲醇-水 7（5:25，体积／体积）； 紫外检测波长205nm，甜菊甙最小检知量：0.2g。 分析了18个甜菊糖甙样品，定量测定结果：甜菊甙含量在43.8060.39%，相对标准偏差为 1.6 。 甜菊糖甙是从菊科植物 甜叶菊的叶 对于甜菊糖甙的分析，国内外报导有重 中提取的多种糖甙盎混合物。其中主要成分是 量法 3〔、气相色谱法 7〔薄层法5,6〕和液