高效液相色谱荧光法同时测定血清中的色氨酸和酪氨酸.pdfVIP

! 756! 2010 10 25 10 LaboratoryMedicine, October2010, Vol25. No 10. :2010) : Q517 : A 任亚萍, 唐爱国, 周前选, 项忠元 (, 410011) : (HPLC)( FD)(Trp) (Tyr) : MegresC18( 250 mm 4. 6 mm, 5 m), 10 , 1. 2 mL/m in, TyrTrp ex = 228nmex = 285 nm em = 306 nm Tyr Trp Tyr em = 353 nm 5 Trp Tyr 3. 4 m in, 0. 275~ 275 mol/L, 0. 004 mol/L, 90. 5 ~ 108. 8 Trp 7. 6m in, 0. 490~ 196mol/L, 0. 005mol/L, 88. 8 ~ 97. 2 Tyr Trp 5 , 5- 20 , TyrTrp, : ;; ; H igh perform ance liquid chroma tographyfluorescence detection for the determ ination of tryptophan and tyrosine in serum REN Yap ing, TANG A iguo, ZHOU Qianxuan, XIANG Zhongyuan. (D epart ent of Clinical Laboratory, the Second X iangyaH osp ital of Central South University, H unan Changsha 410011, China) Abstract: Objective To develop amethod fordeterm ination of tryptophan(Trp) and tyrosine (Tyr) in serum by high performance liquid chromatographyfluorescencedetection (HPLCFD). M ethods The chromatography conditions were aMegres C18 column ( 250mm 4. 6 mm, internal diameter: 5 m), mobile phase ( 10 acetonitrile), flow rate: 1. 2mL/min and the excitation and em ission wave lengths ofTyr and Trp: ex = 228 nm, ex = 285 nm and Tyr Trp em = 306 nm, em = 353 nm. The deproteinized serum samples by 5 ( v/v) perchloric acid solution were Tyr Trp separated and determ ined. The storage condition of samplewas also studied. R esults The retention tmi es ofTyr and Trpwere3. 4 min and 7. 6m in. The linearity rangeswere0. 275275 mol/L and 0. 490196 mol/L. The detection lmi itswere 0. 004 mol/L a