β甘露聚糖酶补料分批发酵条件优化.pdfVIP

地衣芽孢杆菌 HDYM-04 产 β-甘露聚糖酶 补料分批发酵条件优化 解 鑫 平文祥 葛菁萍∗ (微生物黑龙江省高校重点实验室，黑龙江大学生命科学学院，哈尔滨 150080) 摘 要：利用地衣芽孢杆菌(Bacillus licheniformics)HDYM-04 在 5L 发酵罐中发酵生产 β-甘露聚糖酶，并对 其发酵条件、补料策略及用量进行了优化，得到最优起始魔芋粉加入量、初始 pH 值和接种量分别为 60g/L、 8.0 和 6.7% (v/v)，最佳发酵工艺为：温度 37℃，搅拌速率 300r/min,通气量 3L/min，发酵48h 。最后确定最 佳补料策略为起始加入 30g 魔芋粉，对数生长后期再加入 90g 魔芋粉，最终酶活力可高达 3913U/mL，较未 优化前酶活力提高了 89%。 关键词：地衣芽孢杆菌；β-甘露聚糖酶；条件优化；补料发酵；补料策略 中图分类号：Q815 文献标识码：A 文章编号： Optimization of fed-batch fermentation conditions on production of β-mannanase fromBacillus licheniformics HDYM-04 ∗ XIE Xin, PING Wen-xiang , GE Jing-ping (Key Laboratory of Microbiology of Heilongjiang Province,Life Science College,Heilongjiang University,Harbin 150080,China) Abstract: β-mannanase is a kind of hemicellulose enzyme that of great economic value and social significance. It can be mass cultivated in industrial fermenters by using batch fermentation process. The fermentation conditions and feeding strategy to produce β-mannanase from Bacillus licheniformics HDYM-04 were investigated in 5 L fermentor in this study. The optimal initial dosage of konjac powder,initial pH value and inoculum concentration were obtained as 60g/L,8.0 and 6.7% (v/v), respectively. Besides, the optimal conditions of fermenter,such as temperation,agitation speed and aeration rate, were attained as 37 ℃，300r/min and 3L/min,respectively.And the course of fermentation was ended with 48h.At last,a feeding strategy that starting with 30g konjac powder, then adding 90g konjac powder at Logarithmic growth stage was also obtained on the basis of the former conditions. Consequently, the highest enzyme activ