麦长管蚜单克隆抗体及其特性研究.pdfVIP

中国农业科学 2006,39(2):319-323 Scientia Agricultura Sinica 麦长管蚜单克隆抗体及其特性研究 1 内蒙古农业大学农学院 呼和浩特 010019 2 浙江大学生物技术研究所 杭州 310029 3 内蒙古农业大学动物科学与医学学院 呼和浩特 010018 摘要 关键词 The Monoclonal Antibodies to the English Wheat Aphid, Sitobion avenae (Fabricius), and Their Characteristics 1 1 2 1 3 PANG Bao-ping , GAO Shu-jing , CHEN Zheng-xian , ZHOU Xiao-rong , DU Ya-nan 1 2 ( College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019; Institute of Biotechnology, Zhejiang University, Hangzhou 310029 ; 3College of Animal Science and Medicine, Inner Mongolia Agricultural University, Hohhot 010018) Abstract: Objective The English grain aphid, Sitobion avenae (Fabricius), is one of main wheat pests all over the world. It is very important to fully exert the natural control of predatory natural enemies in wheat fields in order to control the wheat aphid. Method Hybridoma technolgy was used to develope monoclonal antibodies(McAbs) to the English grain aphid. The classes and subclasses of McAbs were determined by a double-antibody sandwich ELISA. Antibody specificity was tested using an indirect ELISA. SDSand Western blot analysis were used to determine the proteins reacted with the antibodies. Results Two highly specific monoclonal antibodies were developed, namely EGA-1E8 and EGA-4H11, respectively. McAb EGA-1E8 was IgM subclass while McAb EGA-4H11 belonged to IgG2b subclass. McAb EGA-4H11 reacted with a single peptide of molecular weight 54.2 kD. The specificity test showed