鸡H-FABP基因多态性及其与屠宰性能的关联分析.pdfVIP

HEREDITAS (Beijing) 2007 2 , 29(2): 230―234 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0230 H-FABP 游小燕, 刘益平, 朱庆, 杨志勤 , 625014 : 以 90 日龄草科鸡为实验材料, 对 H-FABP 基因进行 PCR 扩增, 采用 PCR-SSCP 技术结合测序分析了 H-FABP 基因在草科鸡中的多态性。结果表明: 草科鸡中存在332 G →A 、534 G →A 、835 C →T 、1131 →A 、1294C →A 、2329C →T 、2372C →T 、2636C →T 等8 处突变。经过基因型与屠宰性能的关联分析得知: 在7 对产生多 态的引物中, 只有第 1 对引物的各基因型与活重、屠体重、胸肌重、腿肌重和半净膛重差异显著(P0.05); 全净 膛重在不同基因型间差异极显著(P0.01) 。 由此推测, H-FABP 可能对于鸡屠宰性能具有很大的影响或与控制 屠宰性能的主基因连锁。 : H -FABP ; PCR-SSCP; 草科鸡; 屠宰性能 Study on SNP of the H-FABP gene and its association with slaughter performance in chicken YOU Xiao-Yan, LIU Yi-Ping, ZHU Qing, YANG Zhi-Qin College of Animal Science and Technology, Sichuan Agricultural University, Ya ’an 625014, China Abstract: In this experiment, Caoke chicken was used to analyze H-FABP gene polymorphism by PCR-SSCP. Results indicated that there were eight mutation sites in Caoke chicken: 332G→A, 534G→A, 835C→T, 1131→A, 1294C→A, 2329C→T, 2372C→T, 2636C→T. In seven pairs of primers that generated polymorphisms, only the first pair resulted in genotypes that showed a significant difference in body weight, carcass weight, breast weight, leg weight and half-eviscerated weight (P 0.05). It implied that H-FABP could have a major effect on slaughter performance or could be linked to gene(s) that significantly affect slaughter performance in chicken. Keywords: H-FABP ; PCR-SSCP; caoke chicken; slaughter performance 1982 , 1,169.2 g, , 40%50%, 1,033.7 g, [1] , , 27.1%, , , 3.76.0 kg, 3