巨龙竹种子_小穗外植体愈伤组织的诱导培养.pdfVIP

种子净度分析台/ * 1, 2 1 1 耿树香 , 普晓兰, 王曙光 ( 11, 650224; 21, 650204) : , , 3/4M S+ 3 mg/L 2, 4- D+ 013 mg/L KT + 25 g/L, MS+ 3mg/L 2, 4- D+ 01 3mg/L KT + 25 g/L; M S+ ( 3~ 5 mg/L) 2, 4- D + 01 3 mg/L KTM S+ 3mg/L 2, 4- D+ 013 mg/L KT; , : ; ; ; MS; : S 7951 9; S 72213 : A : 1672- 8246 ( 2006) 04- 0078- 06 A Study on Callu s Induction Cu lture ofD endroca lam us sin icus 1, 2 1 1 GE G Shu-xiang , PU X iao-lan, WA G Shu-guang ( 11Southwest Forestry College, Kunm ingYunnan 650224, P1R1China; 21Yunnan Academy of Forestry, Kunming Yunnan 650204, P1R1China) Abs tract: Expermi ent of callus induction culture ofD endrocalam us sinicus including medium screening and brown- prevented was conducted by using seed and small bamboo spike as explants1 The expermi ental results showed that the optmi um m edia for callus induction of seeds and sm allbamboo spikewere 3/4MS + ( 3 ~ 5mg/L) 2, 4- D + 013mg/L KT andMS + 3mg/L 2, 4- D + 013mg/LKT respectively, the rate of callus induction achieved 100% 1 The optmi um multiplicationm ediawereMS + ( 3 ~ 5 mg/L) 2, 4- D + 013 mg/L KT andMS + 3 mg/L 2, 4- D + 013mg/LKT respectively1 The expermi ental results also indicated that cultureunder dark cond-i tion, the brown phenomenon could be somewhat prevented1 K ey w ord s: D endrocalam us sinicus; seed; smallbamboo spike; MS; callus culture 20 80, acum inatn) [ 9] (B 1 ulgaris)(Dendrocal- ,