我国49种线状植物病毒分子鉴定及其基因组研究.pdfVIP

33 5 ( 2007) PLANT PROTECTION Vol.33 No.5 ( 2007) 33 49 * ** 陈剑平 , 陈 炯, 郑红英, 林 林, 程 晔, 史雨红, 鲁宇文 (, , 310021) 本研究建立了马铃薯Y 病毒属马铃薯X 病毒属麝香石竹 隐病毒属和葱X 病毒属成员RTPCR 检测和 全基因组扩增技术体系,并成功用于60 多种供试植物病毒的检测诊断利用所建立的技术体系, 从全国18 个省市 42 种作物上采集的病样中鉴定了49 种植物病毒,其中10 种为新种, 11种为国内新记录, 更正了7 种病毒的鉴定和 命名测定了这49种病毒 217 个分离物基因组序列, 并全部在国际基因数据库登录, 占全球登录的植物病毒基因 组序列总数的3.8%,其中27 种植物病毒为基因组全序列,20 种为国际首次报道, 15 种病毒全序列被美国国家生 物技术信息中心( NCBI) 确定为相关病毒的标准序列 植物病毒; 检测; 鉴定; 种类; 基因组 S 432. 41 Molecular characterization and genomic studies of 49 filamentous plant viruses in China Chen Jianping, Chen Jiong, Zheng Hongying, Lin Lin, Cheng Ye, Shi Yuhong, Lu Yuwen ( K ey Laboratory of Virology, M inistry of A griculture and Zhej iang Province , Institute of Virology and Biotechnology, Zhej iang A cademy of A gricultural ciences, H angz hou 310021, China) Abstract RTPCR systems for detection and amplification of whole genome sequences of members of the genusP o tyvirus, P otex virus, Carlavirus and A llex iv irus were established, which has been successfully applied to detect and sequence over 60 different species of plant viruses. By using these techniques, 49 plant viruses from 42 different crop samples collected from 18 provinces over the country werecharacterized, of which 10 werenew species, 11 were new records in China, and 7 were reidentified and renamed. Two hundred and seventeen isolates of these 49 viruses were sequenced and the data, contributing 3. 8% of theentire world output on plant viruses of all genera, were de posited in the international genomic databases. Of thesedata, 27 were full genomic sequences of plant virus sp