油菜抗旱指标筛选及抗旱相关基因的表达分析.pdf

弱的品种。 干旱成活率实验结果表明，在严重干旱条件下，实验前期筛选到的苗期抗旱性强的 品种3、4 、10、16号具有较高的存活率，抗旱性弱的品种15、20号的存活率较低，从而 证明前期油菜抗旱性鉴定方法是可行的。 水分胁迫下，与拟南芥NCED3 、CBF3、ADH1 、ABA2 基因类似的油菜基因表达量 均有所上升，这些基因可能与油菜芽期抗旱性有关。但基因的克隆与抗旱分子机制有待 进一步研究。 关键字：甘蓝型油菜，抗旱性，鉴定指标，RT-PCR IV ABSTRACT Rapeseed is one of the major oilseed crops in China. Drought is the key environmental factors restricting rapesseed growth and its yield. Breeding for drought-tolerant varieties is one of the most efficient way to alleviate yield reduction caused by drought stress. 20 rapeseed cultivars (Brassica napus L.) are studied during germination and seedling stage under drought conditions. The morphological , physiological and biochemical characteristics are tested and analyzed. Identification index of drought- resistance during germination and seedling stage have been established preliminary. 20 rapeseed cultiwars (Brassica napus L.) are taken as material at germination stage.Different concentrations of PEG-6000(0, 5%, 10%, 15%, 20%) are chosen to simulate different drought degrees.The root length, germination length, total length, root/shoot ratio, root fresh weight, germination fresh weight, total fresh weight, root dry weight germination dry weight and total dry weight are measured in simulated drought stress. 20% PEG-6000 is the optimum concentration used tosimulate drought stress according to the ten morphological indexes at germination stage. The length and fresh weight reduce significantly and the root/shoot ratio, dry weight rise significantly at germination stage under drought stress simulated by PEG -6000. Relative activities of SOD, CAT and relative content of soluble protein and proline are correlated with physiological and biochemical chara