肠道病毒71型重症患儿分离株SDLY96的全基因组序列分析.docVIP

 肠道病毒 71 型重症患儿分离株 SDLY96 的全基因组序列分析# 司鲁莹1，温红玲1，高峰2，赵丽1，宋艳艳1，许洪芝1，袁晓晶1，王志玉1** 5 10 15 20 25 30 35 40 （1. 山东大学公共卫生学院病毒学研究室，实验畸形学教育部重点实验室，济南 250012； 2. 山东省临沂市人民医院，山东 临沂 276000） 摘要：目的 对肠道病毒 71 型重症患儿分离株 SDLY96 进行全基因组序列测定及分析，为构 建肠道病毒 EV71 感染性克隆奠定基础。方法 利用 RT-PCR 对 2010 年山东临沂 1 例手足口病 重症患儿标本分离到的肠道病毒 71 型分离株(SDLY96)进行全基因组核苷酸序列测定,并对 基因组序列进行同源性比较及遗传进化分析。结果 SDLY96 株基因组全长 7405 个核苷酸， SDLY96 株与 Fuyang 株 EU703813 的核苷酸及氨基酸序列同源性均最高,整个基因组核苷酸同 源性为 98.4%,氨基酸同源性为 99.1%;基于 VP1 区的基因遗传进化分析显示, SDLY96 株与 Fuyang 株 EU703813 进化关系最为接近。结论 SDLY96 株属 EV71 病毒的 C4 基因亚型的 C4a 分支，与 Fuyang 株的核苷酸及氨基酸序列同源性很高。 关键词：肠道病毒 71 型；全基因组；序列分析 中图分类号：R117 Sequencing and analysis of complete genome of enterovirus 71 strains isolated from a severe case（SDLY96）in Linyi, Shandong province, 2010 SI Luying1, WEN Hongling1, GAO Feng2, ZHAO Li1, SONG Yanyan1, XU Hongzhi1, YUAN Xiaojing1, WANG Zhiyu1 (1. Dept. of virology, School of public health, Shandong University, JiNan 250012; 2. Linyi People Hospital, ShanDong LinYi 276000) Abstract: Objective To sequence and analyze the complete genome of an enterovirus 71 isolated strain SDLY96 isolated from a severe case, and to lay the foundation for constructing infectious clone of EV71. Methods The SDLY96 complete genomes were segmented amplified by RT-PCR. The homology of nucleotide and amino acid sequences were aligned , and phylogenetic relations of the sequences were analyzed. Results The full length of SDLY96 strains was 7405bp. SDLY96 strain shared the high homology with Fuyang strain（EU703813）both in nucleotide and in amino acid sequences, with 98.4% identity in nucleotides and 99.1% in amino acids. The full VP1 region based phylogenetic analysis also indicated that strain SDLY96 had the close genetic relationship with Fuyang strain（EU703813）. Conclusion SDLY96 should be classified as C4a branch of subgenogroup C4．SDLY96 strain have close genetic relationship with Fuyang strain. Keywords: Enterovirus 71; Complete genome; Sequence analysis 0 引言 EV71 是引起手足口病的主要病原体之一, 与柯萨奇病毒