产蛋前期和产蛋期鹅HPG轴褪黑素受体mRNA表达规律的研究.docVIP

 产蛋前期和产蛋期鹅 HPG 轴褪黑素受体 mRNA 表达规律的研究# 康波，姜冬梅，何珲* 5 10 15 20 25 30 35 40 45 （四川农业大学动物科技学院，四川 雅安 625014） 摘要：为阐明不同繁殖阶段鹅 HPG 轴褪黑素受体 mRNA 表达的变化规律，应用实时荧光定 量 PCR 技术检测产蛋前期和产蛋期四川白鹅下丘脑、垂体、卵巢组织中 3 种褪黑素受体亚 型（Mel-1a、Mel-1b 和 Mel-1c）mRNA 相对表达量。结果表明：产蛋前期鹅下丘脑 Mel-1a、 Mel-1b 和 Mel-1c 的相对表达量分别是产蛋期的 5.25 倍（P0.01）、0.43 倍（P0.01）和 1.20 倍；产蛋前期鹅垂体 Mel-1a、Mel-1b 和 Mel-1c 的相对表达量分别是产蛋期的 3.01 倍 （P0.01）、7.51 倍（P0.01）、7.25 倍（P0.01）；产蛋前期鹅卵巢组织中 Mel-1a、Mel-1b 和 Mel-1c 的相对表达量分别是产蛋期的 1.55 倍（P0.05）、0.55 倍（P0.01）、2.86 倍（P0.01）。 以上结果提示，褪黑素可通过其特异性受体的介导在 HPG 轴的各级水平发挥其调节作用， 并且下丘脑褪黑素主要通过 Mel-1a 介导、垂体褪黑素主要通过 Mel-1b 和 Mel-1c，卵巢褪黑 素主要通过 Mel-1a 和 Mel-1c 介导来发挥其调控作用。 关键词：四川白鹅；褪黑素；下丘脑-垂体-性腺轴；受体；实时荧光定量 PCR 中图分类号：S835 Dynamic regulation of melatonin receptors mRNA expression in the hypothalamus, pituitary gland and ovary of prelaying and laying geese KANG Bo, JIANG Dongmei, HE Hui (College of Animal Science and Technology, Sichuan Agricultural University, SiChuan YaAn 625014) Abstract: In order to elucidate the dynamic regulation of melatonin receptors mRNA expression in the hypothalamic-pituitary-gonadal axis of prelaying and laying Sichuan white geese. The expression levels of Mel-1a, Mel-1b and Mel-1c in the hypothalamus, pituitary gland and ovary samples were determined by quantitative real-time PCR in prelaying and laying Sichuan white geese. The results showed that the expression of Mel-1a, Mel-1b and Mel-1c mRNA in the hypothalamus of prelaying geese was 5.25 (P0.01), 0.43 (P0.01) and 1.20 (P0.05) folds compared with laying geese, respectively. The level of Mel-1a, Mel-1b and Mel-1c mRNA expression in the pituitary gland of prelaying geese was 3.01 (P0.01), 7.51 (P0.01) and 7.25 (P0.01) folds compared with laying geese, respectively. The mRNA expression of Mel-1a, Mel-1b and Mel-1c in the ovary of prelaying geese was 1.55 (P0.05), 0.55 (P0.01) and 2.86 (P0.01) folds compared with laying geese, respectively. These results support the possibility that melatonin mediated the biological function of HPG axis t