城市污水厂污泥脱水液培养小球藻的研究.docVIP

 城市污水厂污泥脱水液培养小球藻的研究 郭志博1,2，潘涌璋1,2** （1. 暨南大学理工学院，广州 510632； 5 10 2. 广东省高校水土环境毒害性污染物防治与生物修复重点实验室，广州 510632） 摘要：分别采用混合不同比例污泥脱水液的培养基培养淡水小球藻，在光照培养箱和光生物 反应器中考察了温度、光照强度以及通气速率，二氧化碳浓度等培养条件对小球藻生长的影 响，结果表明，小球藻在温度为 25、通气速率为 1000mL/min、光照强度 5 级（8310lx）、 二氧化碳浓度 5%的条件下能够更好的生长；小球藻在多组混合培养基中生物量都有一定程 度的增长，但在 100%的污泥脱水液中接种的小球藻生物量增长缓慢，可见用污泥脱水液完 全取代传统培养基培养小球藻，尚不能达到理想的效果，而在污泥脱水液和传统培养基混合 比例为 4:6 条件下，小球藻生长最好。 关键词：环境生物学；污泥脱水液；小球藻 中图分类号：X17 15 A Study for Culturing Chlorella with Sludge Liquor from Municipal Wastewater Treatment Plants GUO Zhibo1,2, PAN Yongzhang1,2 (1. Institute of technology,Jinan university, GuangZhou 510632; 20 25 30 35 40 2. Key Lab.Water/Soil Toxic Pollutants ControlBioremediation,Dept.Edu.Guangdong Province, GuangZhou 510632) Abstract: The different proportions sludge liquor fluid medium respectively was used to culture freshwater chlorella, This article studies the culture conditions including temperature, light intensity ,aeration rate and the concentration of carbon dioxide,how to influence the growth of chlorella in the light incubator and light bioreactor. The results show that, The optimal growth conditions of chlorella was founded, the optimal temperature is 25℃, the optimal ventilation rate is 1000mL/min, the optimal illumination intensity is 5 leval（8310 lx）, the concentration of carbon dioxide is 5%; The biomass of chlorella have a certain degree of growth in many groups of mixed medium, but the biomass of chlorella have a slow growth in the simple excess sludge filtrate, it is thus clear that it can not to achieve an ideal effect to use excess sludge filtrate to replace the traditional medium culture completely to cultivate chlorella, The optimal proportion about the mixture including sludge liquor and the traditional medium culture is 4:6. Keywords: Environmental Biologyey; sludge liquor; chlorella 0 引言 中国工程院《中国能源中长期(2030-2050)发展战略研究》课题研究结果表明：我国石油 缺口日益增大，从能源安全的角度考虑，将进口依存度控制在 60%时，2020 年需替代石油 2000 万吨，2030 年的替代量则为 7000 万吨，而目前生物质能源是最佳的替代选择之一，发 展生物质能源