蜂蜜治疗糖尿病创面及对局部血管内皮生长因子与表皮生长因子表达的影响.docVIP

医学研究生学报2013年6月第26卷第6期 JMed Postgra,Vol.26,No.6,June,2013 ·581 · 论 著 (基础研究) 蜂蜜治疗糖尿病创面及对局部血管内皮生长因子 与表皮生长因子表达的影响 杨淑盈，张 谦，单 岩，郑 鑫 [摘要] 目的 蜂蜜可以促进包括糖尿病创面在内的多种创面愈合，具体机制尚不明确。文中探讨蜂蜜治疗糖尿病大 鼠创面的效果并观察创面局部血管内皮生长因子(vascular endothelial growth factor,VEGF)、表皮生长因子(epidermal growth factor,EGF) 的表达。 方法 63只SD 大鼠随机分为正常组(n=20)、 糖尿病组(n=22) 和蜂蜜组(n=21)。 在大鼠模型的 背部制造1个全皮层皮肤创面，分别给予不同处理方法，观察3组大鼠的创面愈合情况、创面成纤维细胞(fibroblasts,FB)数 量及VEGF、EGF表达的情况。 结果 蜂蜜组创面愈合时间比糖尿病组明显缩短(P0.05); 蜂蜜组创面组织 VEGF 的早期 表达高于糖尿病组(P0.05);EGF 的表达及FB 增殖速度高于糖尿病组(P0.05)。 结论 局部应用蜂蜜能促进糖尿病 创面的愈合，其作用可能与蜂蜜促进EGF 的表达相关。 [关键词] 糖尿病溃疡；蜂蜜；血管内皮生长因子；表皮生长因子 [中图分类号] R587.1 [文献标志码] A [文章编号] 1008-8199(2013)06-0581- 04 Local application of honey for diabetic wounds and the and its effects on vascular endothelial growth factor and epidermal growth factor expressions in the local tissue YANG Shu-ying,ZHANG Qian2,SHAN Yan, ZHENG Xin3 (1.The Nursing College, 2.Pediatric Surgery Department of the First Affiliated Hospital, 3.Endocrine and Metabolic Diseases Departiment of the First Affiliated Hospial, Zhengzhou University, Zhengzhou 450052,Henan,China) [Abstract ] Objective Honey can promote the healing of a variety of wounds, including diabetic wound, but its action mech- anisms remain unknown. The aim of this study is to observe the effect of honey on diabetic wounds and the expressions of VEGF and EGF in the local wound tissue. Methods Sixty-three SD rats were randomized into three groups: normal control(n=20), diabetes mellitus(n =22)and honey treatment (n=21). A round full-thickness skin wound was made on the backs of the rats, followed by observation of the wound surface, the proliferation of fibroblasts and the expressions of VEGF and EGF. Results The healing time of the wound surface was significantly shorter and the early expression of VEGF markedly higher in the honey treatment than in the diabe- tes mellitus group(both P0.05). The proli