单核细胞趋化蛋白-1和转化生长因子β1及结缔组织生长因子在2型糖尿病肾病大鼠肾组织中的表达.docVIP

·2473 · · 论 著 · 单核细胞趋化蛋白-1和转化生长因子β?及结缔组织生长因子 在2型糖尿病肾病大鼠肾组织中的表达 张亚莉，冯婕，姜莎莎，李燕，孙吉平，王慧芳，于琳华，郭尚武 【摘要】 目的 观察单核细胞趋化蛋白-1 (MCP-1)、 转化生长因子β (TGF- β) 及结缔组织生长因子 (CTGF) 在链脲佐菌素 (STZ) 诱发的2型糖尿病肾病大鼠肾组织中的表达。方法 将35只SD 雄性大鼠随机分为对 照组14只和糖尿病肾病组21只。糖尿病肾病组给予高脂饮食，产生胰岛素抵抗后一次性腹腔注射STZ,35 mg/kg;对 照组给予常规饲料，腹腔注射等量的柠檬酸-柠檬酸钠缓冲液。采用免疫组织化学法检测两组大鼠肾组织3种因子的 表达。结果 MCP-1、TGF- β、CTGF 在 STZ 诱导的2型糖尿病肾病大鼠肾组织中(主要在肾小管)有较强的表达， 糖尿病肾病组大鼠3种因子的平均吸光度分别为(0.29±0.04)、(0.20±0.02)、(0.20±0.02),对照组大鼠分别为 (0.21±0.02)、(0.17±0.01)、(0.16±0.02),差异均有统计学意义 (P0.05)。 结论 MCP-1、TGF- β、CTGF 在STZ 诱导的2型糖尿病肾病大鼠肾组织中表达增强，参与了糖尿病肾病早期的发病。 【关键词】 单核细胞趋化蛋白1;转化生长因子β;结缔组织生长因子；糖尿病肾病；模型，动物 【中图分类号】 R589.1R692.9 【文献标识码】 A doi:10.3969/j.issn.1007-9572.2013.07.086 张亚莉，冯婕，姜莎莎，等.单核细胞趋化蛋白-1和转化生长因子β;及结缔组织生长因子在2型糖尿病肾病大 鼠肾组织中的表达 [J]. 中国全科医学，2013,16(7):2473-2476. [www.chinagp. net] Expression of MCP-1,TGF-β and CTGF in the Renal Tissues of Rats with Type 2 Diabetic Nephropathy ZHANG Ya -li,FENG Jie,JIANG Sha-sha, et al. Department of Nephrology, the First Affliated Hospital,Medical School of Xian Jiao- tong University,Xian 710061,China 【Abstract] Objective To investigate the expression of monocyte chemotactic protein- 1(MCP- 1), transforming growth factor beta:(TGF-β)and connective tissue growth factor(CTGF)in the renal tissues of rats with STZ induced type 2 diabetic nephropathy. Methods Thirty -five Sprague -Dawley male rats were randomly divided into control group(N group, 14 cases)and diabetic nephropathy model group(DN group, 21 cases).The DN group include rats with insulin resistance in- duced by diets, the rats were given a single intraperitoneal injection of streptozotocin of STZ. The rats in N group were given con- ventional feed, and were given a single intraperitoneal injection of the same amount of citric acid - sodium citrate buff- er. Immunohistochemisty were performed to examine the expression levels of three factor in renal tissue. Results The expression levels of MCP- 1,TGF-β, and CTGF were strong in t