扩张型心肌病并发心力衰竭患者中micrornas的分布.docxVIP

扩张型心肌病并发心力衰竭患者中micrornas的分布 肝不好（hf），随机可接近或功能异常的减轻，而a-cli帕金氏合金氏合金氏合金氏合金氏合金氏合金氏合金氏家族拥有高度的莫哈林蒂和莫哈林蒂。 Arachidonic acid (AA) is one of the most important polyunsaturated fatty acids (PUFAs) present in the plasma membrane of cells.AA can be obtained from the animal products in diet,such as fish,meat,eggs,etc Micro RNAs (miRNAs) are a class of～22 nt long single strand noncoding RNAs which mainly function by pairing with mRNAs to repress their translation or induce degradation In this work,we compared the expression of miRNAs from the heart tissues of DCM-mediated HF patients with those of healthy donors using two public datasets,identified differentially expressed (DE) miRNAs putatively targeting genes in the AA metabolic pathways,and analyzed the distribution of known single nucleotide polymorphisms (SNPs) within the sequences of DE miRNAs.Our study would provide clues for further functional studies of miRNAs in DCM-mediated HF. 1 杏仁杏仁 1.1 enephe-ene的平台 The miRNA-seq datasets used in this study (GSE53081and GSE135055) were downloaded from the Gene Expression Omnibus (GEO) database (/geo/).The GSE53081 dataset was generated from the left ventricle heart tissues of 34 HF patients (21 patients with DCM-mediated HF) and 8healthy donors removal of 3′adaptor using the Cutadapt program(version 2.10) 1.3 tam.5.4与trtesholds/adjsting五轴相关定义/属性表1 The DE miRNAs between samples from the HF patients and healthy donors were identified using R package DESeq2 with fold change≥1.5 and adjusted P value0.05 (Benjamini-Hochberg adjustment) as the thresholds. Functional enrichment analysis of DE miRNAs was performed using online tool TAM 2.0 (/tam2/) 1.4 fdemirnaswrepedictingmora使用网络以fdemirdb The target genes of DE miRNAs were predicted by miRDB (/) 1.5 inedmir-随机性分布 SNPs in DE miRNAs were obtained from the miR-NASNP-v3 database (/miRNASNP/#!/) 2 产品系统 2.1 alityraftingalityraft国际习惯法sificien To identify miRNAs with dysregulated expression in DCM-mediated HF patients,we first collected the small